南方农业学报2024，Vol.55Issue(7)：1981-1991,11.DOI:10.3969/j.issn.2095-1191.2024.07.011
基于高通量测序分析槲皮素对家蚕生长、产丝量及肠道菌群多样性的影响
Effects of quercetin on the growth,silk yield and gut microbiota diversity in silkworms based on high-throughput sequencing
摘要
Abstract
[Objective]This study employed high-throughput sequencing to evaluate the effects of quercetin on the growth,silk yield,and gut microbiota diversity of silkworms,to elucidate quercetin's regulatory function on the silk-worm gut microecology,thereby providing a theoretical foundation for the high-quality production of functional silk.[Method]A total of 240 silkworms of the 9 Fu×7 Xiang breed were selected and divided into 3 groups:a control group(NC group)fed with ordinary mulberry leaves,a low-dose quercetin group(LQR group)fed with mulberry leaves con-taining 1.25%(w/w)quercetin,and a high-dose quercetin group(HQR group)fed with mulberry leaves containing 5.00%(w/w)quercetin.Beginning at the fifth instar(day 1 of the fifth instar),the silkworms were fed continuously for 6 d.The average growth rate,survival rate and cocoon quality indexes were measured,the silkworms'gut microbiota compo-sition was analyzed via high-throughput sequencing.[Result]After quercetin was supplemented into the silkworms'diet,their average growth rate slowed down,and survival rate decreased.The cocoon mass,cocoon shell mass,and rate of co-coon shell in the HQR group were significantly lower than those in the NC group(P<0.05,the same below).The addition of quercetin enhanced the richness and diversity of the silkworm gut microbiota,with a significant difference in species composition observed between the HQR and NC groups.At the phylum level,the dominant bacterial phyla in all groups were Proteobacteria,Cyanobacteria,and Firmicutes.Compared with the NC group,the relative abundance of Proteobac-teria in the HQR group was significantly reduced.At the genus level,the dominant bacterial genera in all groups were Achromobacter,Methylobacterium,norank_f__norank_o__Chloroplast,Sphingomonas and Acinetobacter.The relative abundance of Achromobacter in the HQR group was significantly reduced compared with the NC group.The KEGG signal pathway analysis results showed that the proportion of gut microbiota in the HQR group which annotated to global and overview maps as well as energy metabolism pathways was significantly higher than in the NC group.In contrast,the pro-portion that annotated to amino acid metabolism and membrane transport pathways was significantly lower than in the NC group.Similarly,COG functional annotation analysis revealed that the HQR group had a significantly lower proportion of annotations related to amino acid transport and metabolism,energy production and conversion,and inorganic ion trans-port and metabolism compared to the NC group.These findings aligned with the KEGG pathway analysis,suggesting that silkworms in the HQR group needed to expend more energy to sustain normal growth,while protein biosynthesis was hin-dered,negatively affecting both silkworm growth and silk protein synthesis.[Conclusion]The addition of quercetin to mulberry leaves alters the composition of the silkworm gut microbiota,reducing the relative abundance of Proteobacteria and Achromobacter.This shift leads to an increase in energy metabolism,but a reduction in the efficiency of amino acid metabolism,energy production and conversion,and membrane transport,ultimately impairing silkworm growth and re-ducing silk yield.关键词
家蚕/槲皮素/肠道菌群/代谢功能/高通量测序Key words
silkworm/quercetin/gut microbiota/metabolic function/high-throughput sequencing分类
畜牧业引用本文复制引用
陈文凯,钟杨生,李红霞,陈芳艳..基于高通量测序分析槲皮素对家蚕生长、产丝量及肠道菌群多样性的影响[J].南方农业学报,2024,55(7):1981-1991,11.基金项目
国家自然科学基金面上项目(31970463) （31970463）
广东省现代农业产业技术体系(蚕桑)创新团队建设项目(2022KJ124) General Project of National Natural Science Foundation of China(31970463) （蚕桑）
Guangdong Mo-dern Agricultural Industry Technology System(Sericulture)Innovation Team Development Project(2022KJ124) （Sericulture）
