南方农业学报2024，Vol.55Issue(7)：2031-2043,13.DOI:10.3969/j.issn.2095-1191.2024.07.015
不同方法提取的山茱萸多糖品质特征
Quality characteristics of Cornus officinalis Sieb.et Zucc.polysaccharides extracted by different methods
摘要
Abstract
[Objective]The effects of different extraction methods on the quality characteristics of Cornus officinalis Sieb.et Zucc.polysaccharides were studied to provide reference for the deep processing and development and utilization of C.officinalis polysaccharides.[Method]Six methods were used to extract C.officinalis polysaccharides,including hot water extraction,microwave-assisted extraction,ultrasonic-assisted extraction,acid extraction,alkaline extraction and composite enzyme extraction,and the effects of the different extraction methods on the yields of C.officinalis polysaccha-rides,the contents of total sugars,antioxidant capacity,physicochemical properties,and structural characterization of C.officinalis polysaccharides were compared.[Result]The quality characteristics of C.officinalis polysaccharides obtained by different extraction methods were different.The extraction rate(15.13%)total sugar content(93.43%),ferric ion re-ducing ability(1.31 mmol/g),and C=O and C-H contents in polysaccharides obtained by alkaline extraction method were higher than those obtained by other methods;the oil absorption(19.00 mL/g)and foam stability(41.67%)of C.officina-lis polysaccharides extracted by microwave-assisted extraction were higher than those obtained by other methods;the cho-lesterol adsorption capacity(621.80 mg/g)of the polysaccharides extracted by composite enzyme extraction method was the strongest,and the scavenging rate of 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)radical(60.06%)was greatly higher than that of the other methods;when the mass concentration of C.officinalis polysaccharide extracted by hot water extraction was 0.2 mg/mL,the scavenging rate of 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt(ABTS)free radical(61.20%)was greatly higher than that of other methods.The foaming ability of polysaccharides extracted by ultrasonic-assisted extraction(17.00%)was significantly higher than that of other methods(P<0.05).In addition,Fourier transform infrared spectroscopy showed that the contents of functional groups in C.officinalis polysac-charides extracted by different methods were different;scanning electron microscopy analysis showed that the surface of C.officinalis polysaccharides extracted by hot water extraction and microwave-assisted extraction were smooth and flat,and the structure of the extracted polysaccharides was tightly aligned;C.officinalis polysaccharides by ultrasonic-assisted extraction and composite enzyme extraction had a strong intermolecular repulsive force;X-ray diffraction suggested that there were obvious and strong absorption peaks at 32º and 45º for C.officinalis polysaccharides extracted by alkaline ex-traction method,while those extracted by hot water extraction method,microwave-assisted extraction method,ultrasonic-assisted extraction method,acid extraction method and composite enzyme extraction method had a similar structure;in the thermal stability analysis,the thermal stability of C.officinalis polysaccharides extracted by alkali extraction method was higher than that of other methods,and the mass loss was about 50.2%at 800℃.[Conclusion]The structure,antioxi-dant activity and physicochemical properties of C.officinalis polysaccharides extracted by various methods are different,but different methods have slight effect on their thermodynamic characteristics,showing a similar trend.It can be deve-loped and applied according to the characteristics of polysaccharides extracted by different methods.关键词
山茱萸/多糖/提取方法/品质特征Key words
Cornus officinalis Sieb.et Zucc./polysaccharides/extraction method/quality characteristics分类
农业科技引用本文复制引用
石云鑫,杨家,潘诗琴,曾诚,郭荣荣,李晓蝶,宋旭琴,杨剑,宋亚..不同方法提取的山茱萸多糖品质特征[J].南方农业学报,2024,55(7):2031-2043,13.基金项目
国家自然科学基金项目(32202854) （32202854）
贵州省基础研究计划(自然科学)项目(黔科合基础-ZK[2023]一般451) （自然科学）
遵义市科学技术局、茅台学院市校联合科技研发资金项目(遵市科合HZ字[2021]308号) （遵市科合HZ字[2021]308号）
茅台学院高层次人才科研启动经费项目(mygccrc[2022]091) National Natural Science Foundation of China(32202854) （mygccrc[2022]091）
Guizhou Basic Research Plan(Natu-ral Science)Project(QKHJC-ZK[2023]Yiban 451) （Natu-ral Science）
Joint Science and Technology Research and Development Project of Zunyi Science and Technology Bureau and Maotai College(Zunshikehe HZ[2021]308) （Zunshikehe HZ[2021]308）
Research Start-up Fund Pro-ject of High-level Talents in Maotai University(mygccrc[2022]091) （mygccrc[2022]091）
