南方农业学报2024，Vol.55Issue(7)：2169-2180,12.DOI:10.3969/j.issn.2095-1191.2024.07.028
互花米草根系促生菌的筛选及其挥发性有机物质鉴定
Screening of growth-promoting rhizobacteria from Spartina al-terniflora and identification of their volatile organic compounds
摘要
Abstract
[Objective]The strains with the ability to release volatile organic compounds(VOCs)to promote plant growth were screened from the rhizosphere bacteria of Spartina alterniflora,and the components of VOCs released were analyzed and identified to provide strain resources and theoretical basis for promoting plant growth.[Method]A total of 85 strains of bacteria were isolated from the rhizosphere soil of S.alterniflora as tested bacteria,and the VOCs growth-promoting strains were screened by co-culture with Arabidopsis thaliana in a two-partition culture dish.To further evalua-te the multiple growth-promoting functions,the entropy method was used to select the best strain.It was classified by morphology,physiological and biochemical features,16S rRNA and gyrB gene analysis.Finally,the VOCs components of the strain were identified by solid-phase microextraction and gas chromatography-mass spectrometry(GC-MS).[Re-sult]Six bacteria that could promote the growth of A.thaliana by releasing VOCs were screened:GXMZU2281,GXMZU2346,GXMZU2401,GXMZU3251,GXMZU3294,GXMZU4388.Among them,GXMZU4388 strain had the ability of solubilizing phosphorus,nitrogen fixation,siderophore and heteroauxin(IAA)production.Compared with the control group,GXMZU4388 strain could greatly promote the growth of tomato under the treatment of 20 times and 100 times diluted bacterial solution,and the fresh weight increased by 144.0%and 186.2%respectively.The comprehensive evaluation scores of entropy method were the highest in GXMZU4388 strain.GC-MS was used to identify that the strain could produce 9 VOCs components,of which 8 components have been reported to have the function of inhibiting bacteria or promoting plant growth.The relative contents of 6-methyl-2-heptanone,5-methyl-2-hexanone,5-methyl-2-heptanone and 3-methyl-2-pentanone were relatively high,which occupied a dominant position.Combined with morphological ob-servation,physiological and biochemical characteristics analysis and molecular biological identification of 16S rRNA and gyrB gene sequences,GXMZU4388 strain was identified as Bacillus safensis.[Conclusion]GXMZU4388 had excellent performance in promoting plant growth in all aspects.It can also significantly exert its ability to promote plant growth at low concentrations,and can release a variety of VOCs that are beneficial to plant growth and bacteriostasis.It has exce-llent application potential in the development of bacterial fertilizer.关键词
互花米草/植物根际促生菌(PGPR)/挥发性有机化合物(VOCs)/沙福芽孢杆菌/拟南芥Key words
Spartina alterniflora/plant growth-promoting rhizobacteria(PGPR)/volatile organic compounds(VOCs)/Bacillus safensis/Arabidopsis thaliana分类
农业科技引用本文复制引用
林子彦,王锟,黄点秋,宁美凤,姜明国,高亚慧,丰景..互花米草根系促生菌的筛选及其挥发性有机物质鉴定[J].南方农业学报,2024,55(7):2169-2180,12.基金项目
国家自然科学基金地区基金项目(32360024) （32360024）
广西自然科学基金青年基金项目(2023GXNSFBA026041) （2023GXNSFBA026041）
广西民族大学引进人才科研启动项目(2019KJQD13) （2019KJQD13）
广西民族大学研究生教育创新计划项目(gxun-chxs2022090) Regional Project of National Natural Science Foundation of China(32360024) （gxun-chxs2022090）
Youth Project of Guangxi Natural Science Foundation(2023GXNSFBA026041) （2023GXNSFBA026041）
Guangxi Minzu University Introduced Talent Research Start-up Project(2019KJQD13) （2019KJQD13）
Graduate Education Innovation Project of Guangxi Minzu University(gxun-chxs2022090) （gxun-chxs2022090）
