广西植物2024，Vol.44Issue(9)：1649-1668,20.DOI:10.11931/guihaia.gxzw202303024
马缨杜鹃bHLH转录因子家族的鉴定与表达分析
Identification and expression analysis of bHLH transcription factors family in Rhododendron delavayi
摘要
Abstract
Water deficiency is a critical factor that restricts the utilization of Rhododendron delavayi in landscaping.bHLH transcription factors are pivotal regulators of plant growth,development,and stress responses.This study aimed to identify members of the R.delavayi bHLH transcription factor(RdbHLH)family by utilizing the R.delavayi genome file and transcriptome data.Through bioinformatics methods,we analyzed various aspects,including gene structure,conserved motifs,phylogenetic relationships,protein physicochemical properties,cis-acting elements,protein interaction networks,and expression patterns.The results were as follows:(1)A total of 116 RdbHLH genes were identified,exhibiting significant variations in terms of amino acid composition and molecular weight of proteins.These genes were primarily weakly acidic hydrophilic proteins that function predominantly within the cell nucleus.(2)The RdbHLH family was categorized into 17 subfamilies,where motif structures were conserved within each subfamily but displayed noticeable differences between different subfamilies.Most RdbHLH proteins contained both Motif 1 and Motif 2 at the same time.The promoter region of these genes contained a substantial number of cis-acting elements associated with plant growth and development,hormone response,light response,and stress response.(3)R.delavayi responded to drought stress by activating signal transduction pathways and regulating osmotic pressure and flavonoid biosynthesis systems,leading to stress damage mitigation.Drought stress exerted an impact on the expression of 36 RdbHLH genes,with 12 genes displaying robust induction.Notably,RdbHLH49 and RdbHLH95 might play pivotal regulatory roles in enhancing plant resistance to drought stress.These research findings provide a theoretical basis for further investigations into the biological functions of RdbHLH genes and establish target gene resources for the breeding of superior horticultural varieties of R.delavayi.关键词
马缨杜鹃/bHLH/生物信息学/干旱胁迫/表达模式Key words
Rhododendron delavayi/bHLH/bioinformatics/drought stress/expression patterns分类
农业科技引用本文复制引用
王洪飞,欧静,王孝敬,可珂,范豫,周玉梅,范馨悦..马缨杜鹃bHLH转录因子家族的鉴定与表达分析[J].广西植物,2024,44(9):1649-1668,20.基金项目
国家自然科学基金(32260097). （32260097）
