广西植物2024，Vol.44Issue(9)：1669-1677,9.DOI:10.11931/guihaia.gxzw202304061
基于转录组测序的红树莓bHLH转录因子家族鉴定及分析
Identification and analysis of bHLH transcription factor family in red raspberry based on transcriptome sequencing
摘要
Abstract
bHLH transcription factors are a large family of transcription factors in plants,which play important roles in plant growth and development,secondary metabolism regulation,and hormone response.Red raspberry fruit is rich in raspberry ketone.To investigate the role of bHLH transcription factor in the growth and development of red raspberry and the synthesis of raspberry ketone,members of the red raspberry bHLH gene family were identified and bioinformatics analysis of these genes were performed based on transcriptome sequencing of two red raspberry fruits,'Polka'and'Orange Legend'.The results were as follows:(1)A total of 95 bHLH transcription factor family members were identified in red raspberry fruits.(2)Most of the bHLH transcription factor family members were unstable hydrophobic proteins;and more than half of them were localized in the nucleus.(3)bHLH transcription factor N-terminal contained the conserved His5-Glu9-Arg13 sequence and C-terminal contained the conserved Leu sequence.(4)The phylogenetic tree divided the family members into 20 subfamilies,of which the R subfamily had the most members with 12.(5)The expression pattern map indicated that the bHLH transcription factor was expressed at a higher level during the green fruit stage and at a lower level during the ripening stage.The results provide a basis for screening bHLH transcription factors that are consistent with changes in raspberry ketone content and provide a reference for further exploring the function of red raspberry bHLH family.关键词
红树莓/转录组测序/bHLH转录因子/基因家族/树莓酮Key words
red raspberry(Rubus idaeus)/transcriptome sequencing/bHLH transcription factors/gene family/raspberry ketone分类
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贺志敏,张峻鑫,于丽平,胡康,姬凤岐,黄体冉,杨明峰,马兰青..基于转录组测序的红树莓bHLH转录因子家族鉴定及分析[J].广西植物,2024,44(9):1669-1677,9.基金项目
北京市属高等学校高水平教学创新团队建设支持计划项目(BPHR20220211). （BPHR20220211）
