广西植物2024，Vol.44Issue(9)：1707-1720,14.DOI:10.11931/guihaia.gxzw202312006
基于叶绿体基因组解析广西裂果薯与裂果薯的遗传差异及亲缘关系
Characterization of genetic difference and phylogenetic relationship between Schizocapsa guangxiensis and Tacca plantaginea based on chloroplast genome
摘要
Abstract
The taxonomy of Tacca remains is controversial all the time.Schizocapsa guangxiensis is considered to be the same species as Tacca plantanginea,but some taxonomists classify them into different species based on their morphological differences.In order to clarify the genetic differences and phylogenetic relationship between Schizocapsa guangxiensis and Tacca plantanginea,this study conducted high-throughput DNA sequencing of Schizocapsa guangxiensis,assembled a complete chloroplast genome using bioinformatics software,and compared it with the published Tacca plantanginea chloroplast genome.The results were as follows:(1)The chloroplast genome size of Schizocapsa guangxiensis and Tacca plantanginea was 162 149 bp and 160 749 bp,respectively,and they had the same GC content(36.90%).Notably,the gene types and gene amount were exactly the same in these two species,including 89 protein-coding genes and 37 tRNA genes,and 6 rRNA genes.(2)Codon preference analysis showed that there were certain differences in the codon frequencies used by the two species,but they both prefered codons ending in A/T(U).(3)Compared with T.plantanginea,the SSC boundary of Schizocapsa guangxiensis had obvious expansion,which was the main factor leading to the length variant in chloroplast genome between the two species.(4)There were some sequence divergences between S.guangxiensis and Tacca plantanginea in the LSC and SSC regions,especially the intergenic region,which can be exploited as species-specific molecular marker.(5)Phylogenetic results showed that Schizocapsa guangxiensis and Tacca plantanginea had a rather distant genetic relationship.Although Schizocapsa guangxiensis was placed in Tacca,they belong to two different species.This study enriches the genetic information of the chloroplast genome of S.guangxiensis and provides a theoretical basis for species classification,genetic diversity analysis and species protection of S.guangxiensis.关键词
广西裂果薯/蒟蒻薯属/叶绿体基因组/遗传差异/系统发育分析Key words
Schizocapsa guangxiensis/Tacca/chloroplast genome/genetic difference/phylogenetic analysis分类
农业科技引用本文复制引用
麻云英,邓永彪,吕虎强,周倩如,李景剑,陆昭岑,谢文娟..基于叶绿体基因组解析广西裂果薯与裂果薯的遗传差异及亲缘关系[J].广西植物,2024,44(9):1707-1720,14.基金项目
国家自然科学基金(32060351) （32060351）
广西自然科学基金(2021GXNSFAA220032). （2021GXNSFAA220032）
