海南医学院学报2024，Vol.30Issue(18)：1361-1369,9.DOI:10.13210/j.cnki.jhmu.20240531.002
基于PPARγ/NF-κB信号通路探讨四妙勇安汤对脂肪细胞炎症的作用机制研究
A study on the mechanism of action of Simiaoyong'an Decoction on adipocyte inflammation based on PPARγ/NF-κB signaling pathway
摘要
Abstract
Objective:To investigate the effect of Simiaoyong'an Decoction on lipopolysaccharide(LPS)-induced inflammation in adipocytes and its underlying mechanism through the PPARγ/NF-κB signaling pathway.Methods:An adipocyte inflammation model was established by inducing mature adipocytes with LPS.The expression of proteins involved in the adipocyte inflammatory signaling pathway was evaluated after 24 h of intervention by administration of freeze-dried powder of Simiaoyong'an Decoction.The effect of Simiaoyong'an Decoction on adipocytes activity was detected by CCK-8 assay.The expression of IL-6,NLRP3,PPARγ,NF-κB p65,LXRα and GLUT4 by Western blot(WB).Results:CCK-8 results showed that freeze-dried powder of Simiaoyong'an Decoction had no significant effect on the viability of adipocytes in the range of 0～500 μg/mL.The WB results re-vealed that following LPS intervention,the model group exhibited increased intracellular inflammatory factors IL-6 and NLRP3 expression,decreased PPARγ expression,increased NF-κB p65 expression,and decreased LXRα and GLUT4 expression com-pared to the control group.Compared with the model group,Simiaoyong'an Decoction dose-dependently reduced IL-6 and NL-RP3 expression.Additionally,the expression of PPARγ,LXRα and GLUT4 increased,while the expression of NF-κB de-creased.The anti-inflammatory effect of Simiaoyong'an Decoction was correspondingly weakened after administration of the PPARγ inhibitor GW9662.Conclusion:Simiaoyong'an Decoction exerts an inhibitory effect on adipocyte inflammation via the PPARγ/NF-κB signaling pathway.关键词
四妙勇安汤/PPARγ/NF-κB/脂肪细胞/炎症Key words
Simiaoyong'an Decoction/PPARγ/NF-κB/Adipocytes/Inflammation分类
医药卫生引用本文复制引用
张晴玥,许心蕊,冯露,邵瑞洁,王添钰,王霄,娄利霞,吴爱明,聂波..基于PPARγ/NF-κB信号通路探讨四妙勇安汤对脂肪细胞炎症的作用机制研究[J].海南医学院学报,2024,30(18):1361-1369,9.基金项目
This study was supported by National Natural Science Foundation of China(81874446,82274488) 国家自然科学基金面上项目(81874446、82274488) （81874446,82274488）
