海南医学院学报2024，Vol.30Issue(18)：1392-1402,11.DOI:10.13210/j.cnki.jhmu.20240524.001
益气活血方介导巨噬细胞极化影响慢性难愈性创面的机制研究
Research on the mechanism of Yiqi Huoxue formula mediating macrophage polarization to affect chronic refractory wounds
摘要
Abstract
Objective:To explore the mechanism of Professor Huang Feng's empirical formula Yiqi Huoxue Formula(YQHXF)mediated macrophage polarization therapy for chronic refractory wounds(CRW)based on network pharmacology,mo-lecular docking,and cell experiments.Methods:Combining network pharmacology and molecular docking technology,we ana-lyzed and verified the potential targets of YQHXF on CRW.CCK8 was used to detect the effects of YQHXF and LPS on the mac-rophage and proliferation activity of Raw264.7 cells.A RAW264.7 cell inflammation model was established using LPS,and JAK2 signaling pathway activator coumarin a1(C-a1)and YQHXF drug containing serum intervention were added.The groups were as follows:Raw264.7 group(control group),Raw264.7+LPS group(model group),Raw264.7+LPS+YQHXF group,Raw264.7+LPS+YQHXF+C-a1 group,and Raw264.7+LPS+C-a1 group.Flow cytometry was used to detect the polarization of Raw264.7 cells;RT qPCR and ELISA detected of inflammatory factors IL-4,IL-6,IL-13,TNF-α transcription and expres-sion.Western blot was used to detect the JAK2/STAT3 pathway phosphorylation.Results:Network pharmacology and molecular docking studies found that YQHXF may regulate macrophage polarization,regulate the expression of inflammatory factors,and promote the healing of CRW by mediating the JAK2/STAT3 signaling pathway.The results of cell experiments showed that YQHXF can significantly enhance macrophage proliferation activity(P<0.05);In model group and C-a1 group,JAK2 and STAT3 phosphorylation,M1 macrophage ratio,IL-6 and TNF-α transcription and protein expression were significantly higher than the control group(P<0.05);Compared with the model group and C-a1 group,the addition of YQHXF treatment significant-ly reduced JAK2 and STAT3 phosphorylation,M1 macrophage ratio,IL-6,and TNF-α transcription and protein expression lev-els(P<0.05),increased the proportion of M2 macrophages,as well as the transcription and protein expression levels of IL-4 and IL-13(P<0.05).Conclusion:YQHXF may enhance macrophage proliferation activity,inhibit JAK2 and STAT3 phosphoryla-tion,positively regulate macrophage M2 anti-inflammatory phenotype polarization,inhibit M1 pro-inflammatory phenotype polar-ization,alleviate inflammatory response,and achieve therapeutic effects.关键词
名医经验/慢性难愈性创面/网络药理学/分子对接/JAK2-STAT3/巨噬细胞极化Key words
Experience of renowned doctors/Chronic refractory wounds/Network pharmacology/Molecular docking/JAK2-STAT3/Macrophage polarization分类
医药卫生引用本文复制引用
任悦怡,陈鑫球,张芝桐,姜自伟,董航,黄枫..益气活血方介导巨噬细胞极化影响慢性难愈性创面的机制研究[J].海南医学院学报,2024,30(18):1392-1402,11.基金项目
This study was supported by National Famous Traditional Chinese Medicine Expert Inheritance Studio Construction Project[Guozhongyao Renjiaohan(2022)No.75] （2022）
the National Natural Science Foundation of China(82004390) （82004390）
The"Double First Class"and High-Level University Discipline Reserve Talent Cultivation Project of Guangzhou University of Traditional Chinese Medicine 全国名老中医药专家传承工作室建设项目[国中医药人教函(2022)75号] （2022）
国家自然科学基金资助项目(82004390) （82004390）
广州中医药大学"双一流"与高水平大学学科后备人才培育项目 （）
