平菇60Co-γ诱变孢子杂交新种质的创制及其遗传多样性分析OA北大核心CSTPCD

This study aimed to evaluate the radiation sensitivity of different Pleurotus ostreatus strains and determine suitable radiation dosages for swift and precise identifying hybrid descendants,thereby expediting the creation of new germplasm and accelerating the breeding process.Spore suspensions of four distinct Pleurotus ostreatus strains were irradiated with ten different doses of 60Co-γ radiation.The mortality rates were recorded,and monokaryotic spores of mutagenic strains were obtained.Subsequently,hybrid combinations were conducted,and mutation with 60Co-γ and hybrid breeding was performed.Genetic diversity analysis was performed using microsatellite markers(SSR)on a total of 39 materials,which included mutagenized materials and hybrid descendants.The results indicated that the appropriate radiation condition for strain 5178 was 500 Gy for 200 minutes,600 Gy for 240 minutes for strain 9408,700 Gy for 280 minutes for Hui Mei 2,and 400 Gy for 160 minutes for Pleurotus ostreatus T2.We screened 35 robustly growing hybrids and verified through SSR molecular markers that these strains exhibited distinct bands when amplified with 8 sets of primers.This study provided a technical foundation and theoretical framework for rapid and accurate identification of hybrid offspring,as well as for radiation mutagenesis and the hybrid breeding of Pleurotus ostreatus.