核农学报2024，Vol.38Issue(11)：2074-2082,9.DOI:10.11869/j.issn.1000-8551.2024.11.2074
银杏种子发芽相关基因的挖掘与分析
Exploration and Analysis of Germination Related Genes in Ginkgo biloba Seeds
摘要
Abstract
In order to investigate the molecular mechanism of gibberellin(GA)promoting Ginkgo seed germination,this study compared the morphology of Ginkgo embryos and related physiological and biochemical indicators of seeds after 15 days of cultivation on MS medium or MS medium supplemented with 5 mg L-1 GA,Differentially expressed genes(DEGs)were identified using transcriptome sequencing technology.The physiological activities and metabolic pathways associated with the DEGs were analyzed,key genes involved in carbohydrate metabolism pathways were identified,and the functionality of Gb_33733 gene was validated.The results showed that GA significantly increased the length,weight,germination potential,and germination rate of Ginkgo biloba embryos.After 15 days of germination,the starch and protein content in the seeds decreased,while reducing sugar content and alpha amylase activity increased significantly.GO analysis revealed that 179 DEGs under two treatment conditions were mainly enriched in physiological processes such as photosynthesis,light intensity perception,light quality adaptation,and response to biotic and abiotic stress.The KEGG pathway analysis revealed that DEGs were mainly enriched in carbohydrate metabolism,amino sugar and nucleotide sugar metabolism,photosynthesis,mitogen activated protein kinase(MAPK)signaling,nuclear cytoplasmic transport,and energy metabolism pathways.Six important genes involved in carbohydrate metabolism pathways were identified by protein interaction screening.In addition,qRT PCR results confirmed that GA treatment significantly enhanced the expression levels of Gb_33733,Gb_33736,Gb_19037,Gb_01010,Gb_05593,and Gb_35011 genes.Moreover,the heterologous expression of the Gb_33733 gene promotes the germination of Arabidopsis seeds.This study provides a theoretical foundation for further elucidating the molecular mechanism of GA-induced germination of Ginkgo biloba seeds.关键词
银杏/赤霉素/发芽/差异表达基因/转录组测序Key words
Ginkgo/gibberellin/sprouting/DEGs/transcriptome sequencing引用本文复制引用
张斌,袁志辉..银杏种子发芽相关基因的挖掘与分析[J].核农学报,2024,38(11):2074-2082,9.基金项目
永州市指导性科技计划项目(2020-YZKJ-001),湖南省自然科学基金项目(2024JJ7190) （2020-YZKJ-001）
