华西口腔医学杂志2024，Vol.42Issue(5)：572-580,9.DOI:10.7518/hxkq.2024075
包封骨形态发生蛋白2的不同相对分子质量聚乳酸-聚乙醇酸共聚物微囊促进成骨效果的研究
Osteogenic effect of poly(lactic-co-glycolic acid)microcapsules with different molecular weights encapsulating bone morphogenetic protein 2
摘要
Abstract
Objective This study aimed to explore the effects of bone morphogenetic protein 2(BMP-2)encapsula-ted in poly(lactic-co-glycolic acid)(PLGA)microcap-sules with different molecular weights on the osteogenic ability of osteoblasts.Methods PLGA microcapsules with different molecular weights(12 000,30 000)encap-sulating BMP-2,were prepared using a dual-channel mi-croinjection pump.The morphology and structure of the microcapsules were characterized by optical microscopy and scanning electron microscopy.The sustained-release performance of the microcapsules was characterized by phosphate buffered saline immersion method.The cell compatibility of the microcapsules was detected by the Calcein-AM/PI stain-ing and CCK-8 method.The chemotactic effect of BMP-2-encapsulated microcapsules on MC3T3-E1 cells after 48 h of treatment was detected by the Transwell assay.The alkaline phosphatase activity assay and Alizarin Red S staining were used to characterize the effect of microcapsules on the osteogenic ability of MC3T3-E1 cells.Results Both types of mi-crocapsules with different molecular weights exhibited smooth surfaces,as well as uniform and good cell compatibility.The chemotactic effect of the 12 000 microcapsules was outstanding.The 30 000 microcapsules had a longer sustained-release time,and the initial burst release was reduced by approximately 25%compared with the 12 000 microcapsules.In addition,30 000 microcapsules performed better in long-term osteogenesis induction than 12 000 microcapsules.Conclu-sion In this study,the release of BMP-2 is regulated by adjusting the molecular weight of PLGA,and the results indi-cate that 30 000 microcapsules can better induce the long-term osteogenic ability of MC3T3-E1 cells.关键词
聚乳酸-聚乙醇酸共聚物微囊/骨形态发生蛋白2/成骨Key words
poly(lactic-co-glycolic acid)microcapsules/bone morphogenetic protein 2/osteogenesis分类
医药卫生引用本文复制引用
袁莉红,陈晨,马语笛,梁睿贞..包封骨形态发生蛋白2的不同相对分子质量聚乳酸-聚乙醇酸共聚物微囊促进成骨效果的研究[J].华西口腔医学杂志,2024,42(5):572-580,9.基金项目
National Natural Science Foundation of China(81970927) （81970927）
Key Research and Development Program(So-cial Development Project)of Jiangsu Province(BE2022797) 国家自然科学基金(81970927) （So-cial Development Project）
江苏省重点研发计划社会发展项目(BE2022797) （BE2022797）
