华西口腔医学杂志2024，Vol.42Issue(5)：593-608,16.DOI:10.7518/hxkq.2024.2024037
香芹酚水凝胶对牙周炎大鼠牙槽骨保护作用研究
Protective effect of carvacrol hydrogel on the alveolar bone in rats with periodontitis
摘要
Abstract
Objective This study aimed to investigate the protective effect and mechanism of carvacrol hydrogel on the alveolar bone in rats with periodontitis.Methods A thermosensitive hydrogel supported by carvacrol was pre-pared using poloxamer and hydroxypropyl methyl cellulose as matrix.SD rats were randomly divided into blank group,periodontitis group,blank hydrogel group,and low-,medium-,and high-dose hydrogel groups.The periodonti-tis symptoms and the CT structure of the alveolar bone were observed.The changes in liver,spleen,kidney,and peri-odontal tissues were observed.The related indexes of bone metabolism in serum were detected.The expression of os-teoprotegerin(OPG)and nuclear transcription factor-κB(NF-κB)pathway proteins was determined by Western blot.The levels of inflammatory factors were assessed by quantitative reverse transcription polymerase chain reaction(qRT-PCR).Results Carvacrol hydrogel had good slow release,biocompatibility,and cell adhesion.The periodontitis of rats in the carvacrol hydrogel group was significantly alleviated,the expression of OPG protein in gingival tissue was significantly increased(P<0.01),and the levels of receptor activator of NF-κB ligand(RANKL),receptor activator of NF-κB(RANK),NF-κB protein,and inflammatory factors were significantly decreased(P<0.01).Conclusion Car-vacrol hydrogel can regulate the OPG and NF-κB pathways,reduce alveolar bone absorption,and improve periodontal inflammation.关键词
牙周炎/香芹酚/水凝胶/骨保护素通路/核因子-κB通路/牙槽骨吸收Key words
periodontitis/carvacrol/hydrogel/osteoprotegerin pathway/nuclear transcription factor-κB pathway/alveolar bone resorption分类
生物科学引用本文复制引用
周露露,滕念,高甜甜,王洪斌,高翔..香芹酚水凝胶对牙周炎大鼠牙槽骨保护作用研究[J].华西口腔医学杂志,2024,42(5):593-608,16.基金项目
2023"Outstanding Youth"Project of Heilongjiang Province(YQ2023C016) 2023年黑龙江省"优秀青年"项目(YQ2023C016) （YQ2023C016）
