扬州大学学报（农业与生命科学版）2024，Vol.45Issue(4)：11-19,9.DOI:10.16872/j.cnki.1671-4652.2024.04.002
鲍曼不动杆菌双组分系统bfmR基因缺失株构建及其生物学特性分析
Construction of two-component system bfmR gene deletion strain of Acinetobacter baumannii and analysis of its biological characteristics
摘要
Abstract
To study the effect of two-component system bfmR gene on the biological characteristics of Acinetobacter baumannii,the bfmR gene deletion mutant of A.baumannii strain AB43 was constructed using RecA homologous recom-bination and the changes of biological characteristics were observed.The bfmR gene was analyzed by ExPASY-Prot-Param,SMART,SOPMA and SWISS-MODEL.The results showed that the growth rate of bfmR gene deletion mutant(AB43△bfmR)was significantly reduced,the autopolymerization ability and intracellular superoxide dismutase(SOD)ac-tivity of the bacteria were significantly decreased,the biofilm forming ability,adhesion and invasion ability were not sig-nificantly changed,and the sensitivity to ceftriaxone was significantly increased.The bioinformatics analysis results showed that the bfmR protein was a relatively stable hydrophilic protein,alpha-helix was the highest in the secondary structure,accounting for 36.13％of the total.The bfmR gene of A.baumannii strain AB43 was closely related to that of the standard strain ATCC 17978 and ATCC 19606.In conclusion,the construction of A.baumannii AB43△bfmR provides an experimental basis for further exploration of the regulatory mechanism of bfmR gene in the two-component system.关键词
鲍曼不动杆菌/bfmR/基因缺失/生物学特性Key words
Acinetobacter baumannii/bfmR/gene deletion/biological characteristics分类
农业科技引用本文复制引用
周子严,於亭,张毓宸,王艺炀,黄佳媛,郭停停,焦红梅,李国才..鲍曼不动杆菌双组分系统bfmR基因缺失株构建及其生物学特性分析[J].扬州大学学报（农业与生命科学版）,2024,45(4):11-19,9.基金项目
国家自然科学基金资助项目(82373637、82073611、82002186、81471906) （82373637、82073611、82002186、81471906）
江苏省自然科学基金资助项目(BK20231241) （BK20231241）
