扬州大学学报（农业与生命科学版）2024，Vol.45Issue(4)：29-35,56,8.DOI:10.16872/j.cnki.1671-4652.2024.04.004
LGALS1通过PI3K/Akt信号通路促进胃癌血管生成的研究
LGALS1 promotes angiogenesis of gastric cancer through PI3K/Akt signaling pathway
摘要
Abstract
To investigate the mechanism of lectin galactoside-binding soluble 1(LGALS1)gene in the angiogenesis of gastric cancer.The effects of LGALS1 on prognosis and angiogenesis in gastric cancer were analyzed by bioinformatics and immunohistochemistry.Different expression LGALS1 gastric cancer cell lines were constructed.The mechanism of LGALS1 in gastric cancer angiogenesis was studied by Transwell,tubule formation,qRT-PCR and Western blot.The re-sults showed that the expression of LGALS1 was directly proportional to the overall survival of gastric cancer patients.There was a correlation between the PI3K/Akt pathway and LGALS1 gene in the angiogenesis pathway.The expression of LGALS1 in gastric cancer was positively correlated with the expression of CD31.The expression of LGALS1 in gastric cancer cells significantly promoted the proliferation and duct formation of endothelial cells.These promoted the migration of endothelial cells by regulating the expression of p-Akt.In conclusion,LGALS1 gene can promote angiogenesis and tumor progression of gastric cancer,which may be related to the activation of PI3K/Akt signaling pathway.关键词
胃癌/LGALS1/PI3K/Akt信号通路/血管生成/HUVEC细胞Key words
gastric cancer/LGALS1/PI3K/Akt signaling pathway/angiogenesis/HUVEC cells分类
农业科技引用本文复制引用
仇必军,崇杨..LGALS1通过PI3K/Akt信号通路促进胃癌血管生成的研究[J].扬州大学学报（农业与生命科学版）,2024,45(4):29-35,56,8.基金项目
国家自然科学基金资助项目(81802792) （81802792）
