扬州大学学报（农业与生命科学版）2024，Vol.45Issue(4)：42-48,7.DOI:10.16872/j.cnki.1671-4652.2024.04.006
无花果内生菌 Alternaria sp.QDFB-2菌丝体化学成分的研究
Study on the chemical constituents in the mycelium of endophyte Alternaria sp.QDFB-2 from Ficus carica L.
摘要
Abstract
In order to investigate the chemical constituents in the mycelium of Alternaria sp.QDFB-2,an endophytic fungus isolated from the branch of Ficuscarica L.,isolation and purification of the crude extract from the mycelium of Alternaria sp.QDFB-2 was carried out by silica gel column chromatography,Sephadex LH-20 gel column chromatogra-phy,reversed phase column chromatography and high-performance liquid chromatography.The structures of obtained compounds were then determined by modern spectroscopic techniques such as nuclear magnetic resonance spectroscopy(NMR)and mass spectrometry(MS)as well as by comparison with literatures.The in vitro cytotoxicity of the com-pounds was evaluated by MTT method.Seven compounds were obtained from the crude extract of the mycelium from Al-ternaria sp.QDFB-2,including alternariol(1),tentoxin(2),dihydrotentoxin(3),cyclo(Gly-Pro)(4),cyclo(4-hy-droxy-Pro-Pro)(5),cyclo(S-Leu-S-N-methyl-Phe)(6),and uridine(7).The in vitro cytotoxicity evaluation showed that compounds 1 and 7 could inhibit the proliferation of SMMC-7721 cells with IC50 values of 50.47 μg·mL-1(195.62 μmol·L-1)and 42.50 μg·mL-1(174.18 μmol·L-1),respectively.Compound 1 exhibited moderate prolifer-ation inhibitory activity against A549 cells with an inhibition rate of 52.12％at the concentration of 100 μg·mL-1,while the IC50 values of the positive control cisplatin were 3.46 μg·mL-1(11.53 μmol·L-1)and 5.12 μg·mL-1(17.06 μmol·L-1),respectively.The mycelium of Alternaria sp.QDFB-2 contains rich chemical substances,which is worthy of further re-search.关键词
无花果/内生菌/Alternaria sp./化学成分/细胞毒活性Key words
Ficus carica L./endophyte/Alternaria sp./chemical constituent/cytotoxicity分类
农业科技引用本文复制引用
徐力,刘汉山,叶桐,郝增君,王凤舞,梁詠文,申丽..无花果内生菌 Alternaria sp.QDFB-2菌丝体化学成分的研究[J].扬州大学学报（农业与生命科学版）,2024,45(4):42-48,7.基金项目
山东省自然科学基金面上项目(ZR2020MB101) （ZR2020MB101）
扬州市重点研发计划(社会发展)项目(YZ2023068) （社会发展）
扬州大学大学生科创基金资助项目(X20220741) （X20220741）
