扬州大学学报（农业与生命科学版）2024，Vol.45Issue(4)：74-81,8.DOI:10.16872/j.cnki.1671-4652.2024.04.010
基于FOX hunting system技术的蒙古柳NaCl胁迫相关基因挖掘
Mining of NaCl stress-related genes in Salix linearistipularis based on FOX hunting system technology
摘要
Abstract
In order to explore the genes related to NaCl stress in Salix linearistipularis,more than 400,000 yeast monoclones of S.linearistipularis cDNAs were obtained based on the FOX hunting system technology using Saccharomy-ces cerevisiae InVscI as material in this study.The transgenic yeast was treated with NaCl stress and screened to obtain 105 resistant yeasts,then they were carried out resistance test,in NaCl stress medium,from which three were signifi-cantly resistant to NaCl compared to the control.Bioinformatics analysis showed that the cDNA of S.linearistipularis transformed from yeast 3 is a full-length cDNA sequence,including all open reading frames,with a coding region of 318 bp,encoding 106 amino acid residues,containing the DABB structural domain,and having very high similarity to DABB-like proteins of other plants.Further results of resistance analysis of this yeast in media with different abiotic stress treatments showed significant resistance to all tested abiotic stresses.This study provides a experimental basis for further understanding of the molecular mechanisms of salt tolerance in woody plants.关键词
蒙古柳/非生物胁迫/耐盐/DABB结构城Key words
Salix linearistipularis/abiotic stress/salt tolerance/DABB structural domain分类
农业科技引用本文复制引用
王梦文,董睿,李辛琲,金光德,南桂仙..基于FOX hunting system技术的蒙古柳NaCl胁迫相关基因挖掘[J].扬州大学学报（农业与生命科学版）,2024,45(4):74-81,8.基金项目
吉林省高校科学技术研究项目(JJKH20210573KJ) （JJKH20210573KJ）
吉林省自然科学基金资助项目(20230101278JC) （20230101278JC）
