兰州大学学报（医学版）2024，Vol.50Issue(8)：16-24,47,10.DOI:10.13885/j.issn.1000-2812.2024.08.003
基于液相色谱-三重四级杆质谱仪的栀子及水栀子中萜类物质鉴定及成分差异研究
Identification of and investigation into composition differences of terpenoids in Gardenia jasminoides Ellis and Gardenia jasmi-noides var.radicans Makino using liquid chromatography triple quadrupole mass spectrometer
摘要
Abstract
Objective To develop a method for the high-throughput determination of terpenoids in Gardenia jasminoides Ellis and apply it in an investigation of composition differences between Gardenia jasminoides Eills and Gardenia jasminoides var.radicans Makino.Methods A high-throughput qualitation and quantifica-tion strategy was proposed for the terpenoids in Gardenia jasminoides Ellis by combining multiple techniques such as full scanning,product ion scanning,precursor ion monitoring,neutral loss ion scanning,multiple reac-tion monitoring,and dynamic multiple reaction ion monitoring in liquid chromatography-triple quadrupole mass spectrometry,the multivariate analysis methods such as principal component analysis and orthogonal partial least squares-discriminant analysis were further applied for investigating the composition differences.Results A total of 81 terpenoids were identified and relatively quantified in the present study.There was a sig-nificant difference in the content of terpenoids between Gardenia jasminoides Ellis and Gardenia jasminoides var.radicans Makino.Of them,13 different substances could be used as markers for distinguishing Gardenia jasminoides Ellis and Gardenia jasminoides var.radicans Makino.Conclusion The established method is con-venient and accurate,which provides a new idea for the identification and quality evaluation of gardenia,and provides a reference for the rational development and utilization of gardenia resources.关键词
栀子/水栀子/液相色谱-三重四级杆质谱仪/萜类物质/差异标志物Key words
Gardenia jasminoides Eills/Gardenia jasminoides var.radicans Makino/liquid chromatography triple quadrupole mass spectrometer/terpenoids/differential marker分类
医药卫生引用本文复制引用
雒馨怡,饶志,张帆,魏玉辉..基于液相色谱-三重四级杆质谱仪的栀子及水栀子中萜类物质鉴定及成分差异研究[J].兰州大学学报（医学版）,2024,50(8):16-24,47,10.基金项目
甘肃省科学技术厅联合基金资助项目(23JRRA1492) （23JRRA1492）
国家自然科学基金资助项目(82174067) （82174067）
甘肃省卫生健康委员会中医药科研课题资助项目(GZKP-2O22-33) （GZKP-2O22-33）
兰州大学第一医院院内基金项目(ldyyyn2023-34) （ldyyyn2023-34）
