磷对黑木相思增殖苗丛生芽生长发育的影响OA北大核心CSTPCD

To understand the effect of phosphorus on the induction and growth of cluster buds in the proliferating seedlings of Acacia melanoxylon,the tissue-cultured seedlings of clone SR17 as materials was inoculated proliferation medium containing 0.9,1.2,1.6,2.0,2.4,and 2.7 mmol/L KH2PO4,respectively,and the growth and development indexes of cluster buds were investigated after for 1 and 2 cycles of subgeneration,respectively.The results showed that 2.0-2.7 mmol/L KH2PO4 promoted the rapid growth of height,number of cluster bud and base callus of proliferating seedlings,among which 2.4 mmol/L KH2PO4 had the best effect,and the accumulation of biomass and chlorophyll content was higher.With the enhance of phosphorus content in the medium,phosphorus and potassium contents in the cluster buds significantly increased,while nitrogen content had no significant change.Pearson correlation analysis revealed that the phosphorus level had significantly positive correlation with height,number,base callus,fresh weight,dry weight,tissue water content,and contents of total chlorophyll,chlorophyll a,chlorophyll b,phosphorus,potassium of bud in 1 and 2 cycles of subgeneration.Therefore,phosphorus plays an essential regulatory role in the growth and development of cluster buds of A.melanoxylon.