光叶兔耳风石油醚部位化学成分和抗菌活性研究OA北大核心CSTPCD
Chemical Constituents from Petroleum Ether Fractions of Ainsliaea glabra and Antibacterial Activities
为了解兔耳风(Ainsliaea glabra)的化学成分,采用硅胶、凝胶(Sephadex LH-20)柱色谱、半制备高效液相色谱等方法,从其90%乙醇提取物的石油醚萃取部位分离了10个化合物,根据波谱数据分别鉴定为松柏烯醇二异戊酸酯(1)、3β-hydroxy-11α,13-dihydro-costunolide(2)、matricarin(3)、乙酰伪蒲公英甾醇(4)、白桦脂醇(5)、27-hydroxyolean-12-en-28-oic acid(6)、morolic acid acetate(7)、异黑麦草内酯(8)、苯甲酸丁酯(9)和E-phytenal(10),其中化合物1 为新结构化合物,化合物3 和7 首次在兔耳风属中分离得到,化合物 2～7、9 均为首次在光叶兔耳风报道.抗菌活性测试结果表明,化合物 1、2、8 对金黄色葡萄球菌有一定抑制作用,MIC值分别为31.2、25.0、21.8 μg/mL,化合物1 和3 对白色念珠菌有潜在抑制作用,MIC值分别为1.9和3.9 μg/mL.
In order to understand the chemical composition of Ainsliaea glabra,ten compounds were isolated from petroleum ether extraction fraction of 90% ethanol extract by silica gel,sephadex LH-20 gel column chromatography,semi-preparative high-performance liquid chromatography.Based on spectral data,their structures were identified as pinerenol diisovalerate(1),3β-hydroxy-11α,13-dihydro-costunolide(2),matricarin(3),taraxasteryl acetate(4),betulyl alcohol(5),27-hydroxyolean-12-en-28-oic acid(6),morolic acid acetate(7),(-)-loliolide(8),butyl benzoate(9),and E-phytenal(10).Compound 1 was a new structural compound.Compounds 3 and 7 were isolated from Ainsliaea genus for the first time.Compounds 2-7 and 9 were first reported in the A.glabra.The results of antibacterial activity test showed that compounds 1,2 and 8 had certain inhibitory effects on Staphylococcus aureus with MIC values of 31.2,25.0,and 21.8 μg/mL,and compounds 1 and 3 had potential inhibitory effect on Candida albicans with MIC values of 1.9 and 3.9 μg/mL,respectively.
姜国东;张森雨;刘亚凤;安振宇;吴秀丽;王芳;江志波;马晓莉;南泽东
北方民族大学化学与化学工程学院,国家民委化工技术重点实验室,银川 750021宁夏医科大学药学院,银川 750004山东省药学科学院,山东省化学药物重点实验室,济南 250101
光叶兔耳风化学成分结构鉴定抗菌活性
Ainsliaea glabraChemical constituentStructure identificationAntibacterial activity
《热带亚热带植物学报》 2024 (005)
660-666 / 7
宁夏自然科学基金一般项目(2022AAC03292);北方民族大学研究生创新项目(YCX22164);宁夏自然科学基金优秀青年项目(2022AAC05041);宁夏重点研发项目(2021BEB04019)资助This work was supported by the General Program for Natural Science in Ningxia(Grant No.2022AAC03292),the Program for Graduate Innovation of North Minzu University(Grant No.YCX22164),the Program for Outstanding Youth of Natural Science in Ningxia(Grant No.2022AAC05041),and the Program for Key Research&Development in Ningxia(Grant No.2021BEB04019).
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