四川大学学报（自然科学版）2024，Vol.61Issue(5)：223-229,7.DOI:10.19907/j.0490-6756.2024.056002
麻疯树核糖体失活蛋白Curcin和Curcin C的RNA N-糖苷酶活性研究
Study on RNA N-glycosidase activity of ribosome inactivating proteins Curcin and Curcin C of Jatropha curcas
摘要
Abstract
To investigate the RNA N-glycosidase activity of ribosome inactivating proteins in Jatropha cur-cas,the UPLC-MS/MS method was established to determine the in vitro RNA N-glycosidase depurination activity of Jatropha ribosomal inactivating proteins Curcin and Curcin C.The optimal conditions for the reac-tion of Curcin with RNA32 were 37℃,pH=4.2,and 4 h.For Curcin C reacted with RNA32,the optimal conditions were 37℃,pH=3.7 and 8 h.The Km values of Curcin was 8.231 μmol/L,while that of Curcin C was 3.302 μmol/L,indicating a high affinity of Curcin for the substrate.Some of the metal ions had a pro-motive effect on the enzymatic activity of Curcin C,while all produced inhibition in the case of Curcin.When the adenosine concentration reached 250 μmol/L,the enzyme activity of Curcin C increased,whereas it had no effect on Curcin.When the enzymatic reaction substrates were RNA14 and RNA32,the RNA N-glycosidase activity of Curcin C protein was significantly higher than that of Curcin,indicating that Curcin C has more potential for antitumor drug development compared to Curcin.关键词
核糖体失活蛋白/Curcin/Curcin C/RNA N-糖苷酶活性/UPLC-MSKey words
Ribosome-inactivating protein/Curcin/Curcin C/RNA N-glycosidase activity/UPLC-MS分类
生物科学引用本文复制引用
马芮,彭婕,徐莺..麻疯树核糖体失活蛋白Curcin和Curcin C的RNA N-糖苷酶活性研究[J].四川大学学报（自然科学版）,2024,61(5):223-229,7.基金项目
国家自然科学基金(31870315) （31870315）
