四川大学学报（自然科学版）2024，Vol.61Issue(5)：230-236,7.DOI:10.19907/j.0490-6756.2024.056003
AtbHLH059调控拟南芥花粉精细胞发育
AtbHLH059 regulates pollen sperm cell development in Arabidopsis thaliana
摘要
Abstract
To further investigate the biological functions of AtbHLH059,this study identified AtbHLH059 loss-of-function mutant(atbhlh059)and constructed overexpression lines(OEs).The mature pollen from various lines were observed using an electron microscope,and found that some pollen staining with DAPI,had abnormal morphology and no sperm cells in atbhlh059 mutants.Alexander staining and pollen germina-tion experiments confirmed that these pollen grains were sterile.qRT-PCR results revealed that the expres-sion levels of DUO1(duo pollen1),DUO3(duo pollen3),FBL17(f-box-like17),CYCB1(cyclin b1)and GCS1(genera tive cell specific 1),which are sperm cell development marker genes,in atbhlh059 were significantly down-regulated while there was no significant difference in overexpression lines,compared with those in the wild-type plants.In conclusion,AtbHLH059 regulates pollen sperm cell formation and pollen morphology and fertility in Arabidopsis thaliana.关键词
花粉/AtbHLH059/拟南芥Key words
Pollen/AtbHLH059/Arabidopsis thaliana分类
农业科技引用本文复制引用
刘佳佳,吉浩,谢依婷,杨毅,李小意..AtbHLH059调控拟南芥花粉精细胞发育[J].四川大学学报（自然科学版）,2024,61(5):230-236,7.基金项目
四川省自然科学基金(2023NSFSC1275) （2023NSFSC1275）
