山东医药2024，Vol.64Issue(27)：1-5,5.DOI:10.3969/j.issn.1002-266X.2024.27.001
miR-146a-5p、SMAD4在甲状腺滤泡癌组织中表达变化和对FTC-238细胞系增殖迁移侵袭影响及靶向关系
Expression changes of miR-146a-5p and SMAD4 in thyroid follicular carcinoma tissues,their effects on proliferation,migration,and invasion of FTC-238 cells,and verification of the targeted relationship
摘要
Abstract
Objective To analyze the targeted relationship between miR-146a-5p and SMAD4 by observing the ex-pression changes of microRNA-146a-5p(miR-146a-5p)and SMAD4 homolog 4(SMAD4)in follicular thyroid carcinoma(FTC)tissues and their effects on cell proliferation migration and invasion abilities,in order to explore whether miR-146a-5p promotes invasion and metastasis of FTC by targeting SMAD4.Methods We collected 30 samples of FTC tissues and 30 samples of non-tumor thyroid tissues or tissues adjacent to the tumor margin(>2 cm)from patients with FTC.We used RT-qPCR to detect miR-146a-5p and SMAD4 mRNA in the FTC tissues and adjacent tissues.The FTC-238 cells were di-vided into the miR-146a-5p mimics group and the negative control(miR-NC group),which were transfected with miR-146a-5p mimics(to overexpress miR-146a-5p in the cells)and miR-NC,respectively.CCK-8 was used to observe the cell proliferation abilities of the two groups at 12,24,and 48 h of culture.The scratch test was used to observe the migration abilities of the two groups,and the Transwell chamber experiment was used to observe the invasion abilities of the two groups.RT-qPCR and Western blotting were used to detect SMAD4 mRNA and protein in the two groups,respectively.The dual luciferase reporter gene experiment was used to verify the targeted relationship between miR-146a-5p and SMAD4.Results The relative expression level of miR-146a-5p in FTC tissues was lower than that in the adjacent tissues(P<0.05).The relative expression level of SMAD4 mRNA in FTC tissues was higher than that in adjacent tissues(P<0.05).The OD value at each time point in the miR-146a-5p mimics group was lower than that in the miR-NC group(all P<0.05).The number of migration and invasive cells after transfection in the miR-NC group was larger than that in the miR-146a mimics group(P<0.05).The relative expression levels of SMAD4 mRNA and protein in the miR-146a-5p mimics group were lower than those in the miR-NC group(both P<0.05).There was a targeted relationship between miR-146a-5p and SMAD4.Conclusion In FTC tissues,the expression of miR-146a-5p decreases while the expression of SMAD4 in-creases.Overexpression of miR-146a-5p can inhibit the proliferation,migration,and invasion of the FTC-238 cells.There is a targeted relationship between miR-146a-5p and SMAD4.MiR-146a-5p may promote the proliferation,invasion,and migration of FTC cells by targetedly inhibiting SMAD4.关键词
微小RNA-146a-5p/SMAD同源物4/细胞增殖/细胞侵袭/细胞转移/甲状腺滤泡癌Key words
microRNA-146a-5p/SMAD homolog 4/cell proliferation/cell invasion/cell metastasis/thyroid fol-licular carcinoma分类
医药卫生引用本文复制引用
黄睿,刘红春,王昌敏..miR-146a-5p、SMAD4在甲状腺滤泡癌组织中表达变化和对FTC-238细胞系增殖迁移侵袭影响及靶向关系[J].山东医药,2024,64(27):1-5,5.基金项目
新疆维吾尔自治区自然科学基金项目(2023D01C59). （2023D01C59）
