山东医药2024，Vol.64Issue(27)：17-20,4.DOI:10.3969/j.issn.1002-266X.2024.27.004
α-葡萄糖苷酶低表达对胰岛β细胞形态、增殖能力、炎症及氧化应激反应的影响
Effects of low expression of α-glucosidase on morphology,proliferation,inflammatory response and oxidative stress response of pancreatic β cells
摘要
Abstract
Objective To observe the effects of low expression of α-glucosidase(GAA)on morphology,prolifera-tion,inflammatory response,and oxidative stress response of pancreatic β-cells in order to investigate the potential role of GAA in the pathogenesis of diabetes mellitus.Methods The expression of GAA in βTC6 cells was interfered by siR-NA1,2,3 and 4,respectively.As a result,the interference effect of siRNA4 was the best,and it was used as the interfer-ence sequence in subsequent experiments.βTC6 cells were randomly divided into the siRNA group,negative control group(NC group)and blank control group(Con group),respectively.Cells in the siRNA group were transfected with siR-NA4 sequence to reduce the expression of GAA,cells in the negative control group were transfected with siRNANC se-quence,and cells in the Con group were not treated.After 24,48,72 and 96 h of cell culture,the cell proliferation of each group was detected by CCK-8.They were cultured for 48 h,the number and morphology of cells in each group were observed by inverted microscope,the inflammatory factors IL-6 and IL-1β protein in cells were detected by Western blot-ting,and the content of reactive oxygen species(ROS)in cells was detected by immunofluorescence.Results Com-pared with the Con and NC groups,the cells increased,the intercellular space became smaller under the microscope,the OD values of cells increased at 24,48,72,and 96 h of culture,and the levels of IL-6 and ROS decreased after 48 h of cul-ture in the siRNA group(all P<0.01).No significant differences were found in these indicators between the Con and NC groups(all P>0.05).Conclusions Low expression of GAA can lead to alterations in cell morphology,enhanced prolif-erative capacity,and diminished inflammatory and oxidative stress responses of pancreatic β-cells.GAA may participate in the pathogenesis of diabetes by changing the morphology of pancreatic β-cells and enhancing inflammatory and oxidative stress responses.关键词
α-葡萄糖苷酶/胰岛β细胞/βTC6细胞/细胞增殖/炎症反应/氧化应激反应/糖尿病Key words
α-glucosidase/pancreatic β-cells/βTC6 cells/cell proliferation/inflammatory response/oxidative stress response/diabetes mellitus分类
医药卫生引用本文复制引用
庞越胜,罗倩倩,蒋靓,徐逸凡,帕它木·莫合买提..α-葡萄糖苷酶低表达对胰岛β细胞形态、增殖能力、炎症及氧化应激反应的影响[J].山东医药,2024,64(27):17-20,4.基金项目
新疆维吾尔自治区天山雪松计划项目(2019XS03). （2019XS03）
