山东医药2024，Vol.64Issue(27)：40-44,5.DOI:10.3969/j.issn.1002-266X.2024.27.009
含白藜芦醇培养基培养的人眼结膜和胸部皮肤病理性瘢痕成纤维细胞增殖凋亡侵袭迁移情况观察
Proliferation,apoptosis,invasion,and migration of fibroblasts in human conjunctiva and thoracic skin pathological scars cultured in resveratrol-containing medium
摘要
Abstract
Objective To observe the proliferation,apoptosis,invasion,and migration of human ocular conjuncti-val pterygium(ocular pathological scar)fibroblasts and human anterior thoracic skin pathological scars(skin-derived path-ological scars)fibroblasts cultured in resveratrol-containing medium,in order to investigate the effects of resveratrol on the proliferation,apoptosis,invasion,and migration of human ocular pathological scar fibroblasts and skin-derived pathologi-cal scar fibroblasts.Methods Primary human ocular pathological scar fibroblasts and skin-derived pathological scar fi-broblasts were isolated by tissue block adherent method.The cell viability(OD value)of human ocular and skin-derived pathological scar fibroblasts was detected by CCK-8 when the cells were cultured in media containing 0(control),1,2,4,8,16,32,64,128 and 256 µg/mL resveratrol for 48 h.Human ocular pathological scar fibroblasts and skin-derived pathological scar fibroblasts were cultured with 0 and 75 µg/mL resveratrol for 8 h,and the apoptosis rate was measured by Tunel method.These two kinds fibroblasts were cultured in 0 and 30 µg/mL resveratrol media for 48 h,and the migra-tion and invasion abilities of the cells(the number of invasive migratory cells)were observed.Results The OD values of human ocular pathological scar fibroblasts cultured in media containing 128 and 256 µg/mL resveratrol decreased(both P<0.05),and the OD values of skin-derived pathological scar fibroblasts cultured in media containing 64,128,and 256 µg/mL resveratrol all decreased(all P<0.05),in comparison with those at 0 µg/mL resveratrol.Compared with those at 0 µg/mL resveratrol,the apoptosis rates of human ocular pathological scar fibroblasts and skin-derived pathological scar fi-broblasts cultured in 75 µg/mL resveratrol medium increased(all P<0.05),and the number of invasion and migration cells of human ocular pathological scar fibroblasts and skin-derived pathological scar fibroblasts cultured in medium con-taining 30 µg/mL resveratrol became smaller(all P<0.01).Conclusions Human ocular pathological scar fibroblasts and skin-derived pathological scar fibroblasts cultured in resveratrol-containing medium have reduced viability,inva-sion,and migration abilities,and increased apoptosis rate.Resveratrol can significantly inhibit the proliferation,inva-sion,and migration and promote the apoptosis of human ocular pathological scar fibroblasts and skin-derived pathological scar fibroblasts.关键词
白藜芦醇/成纤维细胞/病理性瘢痕/翼状胬肉/细胞增殖/细胞迁移/细胞凋亡Key words
resveratrol/fibroblasts/pathological scar/pterygium/cell proliferation/cell migration/apoptosis分类
医药卫生引用本文复制引用
张艺龄,唐志铭,乔磊,宫朝举,李美丽,庞昆,丁继存..含白藜芦醇培养基培养的人眼结膜和胸部皮肤病理性瘢痕成纤维细胞增殖凋亡侵袭迁移情况观察[J].山东医药,2024,64(27):40-44,5.基金项目
江苏省中医药科技发展计划项目(MS2021051) （MS2021051）
徐州市科技项目(KC21262、KC22160) （KC21262、KC22160）
徐州医科大学附属医院发展基金(XYFM202317) （XYFM202317）
中国博士后基金面上项目(2022M722674) （2022M722674）
徐州市医学后备人才计划项目(XWRCHT20220009). （XWRCHT20220009）
