生物技术通报2024，Vol.40Issue(9)：20-32,13.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0521
木薯镁离子转运蛋白家族基因的鉴定及生物信息学分析
Identification and Bioinformatics Analysis of Gene in the Magnesium Transporter Family in Cassava
摘要
Abstract
[Objective]Magnesium transporters(MGTs)are vital for magnesium transportation,thus analyzing the basic information,the physiological and chemical characteristics of the MeMGT gene family in cassava serves as a foundational step for understanding the functions of these genes.[Method]In this study,we identified and named 13 putative MeMGTs genes in cassava(Manihot esculenta Crantz)based on the cassava genome database through homologous sequence alignments,designated as MeMGT2;1-MeMGT10;2.We conducted chromosomal localization,conserved motif analysis,and phylogenetic analysis of the MeMGT family genes.Additionally,we predicted the biochemical properties,structure,and function of the corresponding proteins.[Result]Chromosomal analysis revealed that the 13 MeMGTs genes were distributed across 8 chromosomes,each containing 4 to 13 exons.Tissue-specific expression patterns indicated differential expressions of MeMGTs genes across various developmental stages.MeMGT4;2 was mainly expressed in cassava fibrous root and storage root,which might potentially play a role in storage root development,and MeMGT7 was mainly expressed in cassava leave,vein and stem with function possibly involved in long-distance Mg2+transport.Cis-acting element analysis revealed the presence of numerous photoresponsive and hormone-responsive elements in the promoter regions of MeMGTs genes,suggesting regulation by multiple factors.Phylogenetic analysis of the MGT gene family categorized these genes into five distinct clusters between cassava and selected model species.Protein sequence analysis identified MeMGTs member proteins as hydrophilic proteins with two conserved transmembrane domains and a Gly-Met-Asn(GMN)tripeptide motif,devoid of signal peptides.Phosphorylation analysis indicated the presence of phosphorylation sites,including serine,threonine,and tyrosine sites,which may contribute to the functionality of MeMGTs member proteins.[Conclusion]MeMGTs genes are regulated by various elements including light,hormone,drought and cold responses,might play a crucial role in the growth,development and adversity stress of cassava.关键词
木薯/镁离子转运蛋白/MeMGTs基因家族/基因鉴定/生物信息学Key words
cassava/magnesium transporter/MeMGT gene family/gene identification/bioinformatics引用本文复制引用
谭博文,张懿,张鹏,王振宇,马秋香..木薯镁离子转运蛋白家族基因的鉴定及生物信息学分析[J].生物技术通报,2024,40(9):20-32,13.基金项目
国家自然科学基金项目(31801417),农业农村部国家现代农业产业技术体系项目(CARS-11) （31801417）
