生物技术通报2024，Vol.40Issue(9)：42-50,9.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0509
马铃薯StDREBs基因的克隆及其表达分析
Cloning and Expression Analysis of StDREBs Gene in Solanum tuberosum L.
摘要
Abstract
[Objective]This study is aimed to investigate the expression characteristics of OsDREB1C homologous genes(StDREBs)in potato(Solanum tuberosum L.)under low nitrogen conditions,evaluating their potential to enhance nitrogen use efficiency in potato,thereby providing a theoretical basis for subsequent gene functional validation and crop improvement.[Method]The homologous gene of OsDREB1C in potato was obtained by homologous cloning.Four homologous genes of OsDREB1C were found in the potato.Their expressions were analyzed via bioinformatics,subcellular localization and low nitrogen conditions.[Result]StDREBs protein in potato contained AP2 conserved domain,and the protein products were located in the nucleus.StDREBs gene was expressed in the roots,stems and leaves,with a higher expression in the roots,followed by in the stems,and the lowest relative expression in the leaves.Under nitrogen deficiency treatment,the expressions of StDREBs gene in different tissues increased with the increasing of treatment time,and the expression of StDREBs gene reached its peak when 1/10N was treated for 18 h,indicating that StDREBs participated in the response to low-nitrogen environment in potato.[Conclusion]OsDREB1C homologs(StDREBs)play a key role in potato under low-nitrogen conditions.关键词
StDREBs/马铃薯/氮胁迫响应/光诱导Key words
StDREBs/potato/response to nitrogen stress/photo-induction引用本文复制引用
张小妹,周南伶,张赛行,王超,沈玉龙,管俊梅,马玲..马铃薯StDREBs基因的克隆及其表达分析[J].生物技术通报,2024,40(9):42-50,9.基金项目
云南省科技厅基础研究专项(202201AT070037),云南省William John Lucas院士工作站(202105AF150028) （202201AT070037）
