生物技术通报2024，Vol.40Issue(9)：51-63,13.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0439
马铃薯AQP基因家族鉴定及表达分析
Identification and Expression Analysis of AQP Gene Family in Potato
摘要
Abstract
[Objective]Aquaporins(AQPs)are crucial in physiological processes such as plant growth and development,water transport and stress response under abiotic stress in plants,and the present study was aimed to reveal the properties and functions of the potato StAQP family.[Method]The potato's StAQP gene family was identified by genome-wide,and their gene structures,conserved motifs,cis-acting progenitors,chromosomal localization,and gene collinearity were analyzed using bioinformatics,and the gene expression patterns were verified by combining transcriptome data and RT-qPCR.[Result]The 44 members of the potato StAQP gene family were distributed in five subfamilies:PIP,TIP,NIP,SIP and XIP.The gene structures and conserved motifs of the members in the same subfamily were basically the same,and the most of the members of the potato AQP family were distributed in the plasma membrane;the members of the potato StAQP family contained a large number of cis-acting progenitors related to light and stress response.The results of collinearity analysis showed that there were a large number of homologous gene pairs in AQP family between the potato and Arabidopsis thaliana,Nicotiana attenuata,Solanum lycopersicu and Capsicum annuum.The results of transcriptome and RT-qPCR validation showed that the potato's StAQP family members were highly expressed under drought and salt stress,and that there was a difference in the expressions of different members under different stresses.Differences in the expression of the same genes under different stresses also existed,three of these genes,StAQP7,StAQP28 and StAQP31,were the key genes of the StAQP family in response to abiotic stresses.[Conclusion]StAQPs genes play an important role in the growth and development of potato and in the response to abiotic stresses.关键词
马铃薯/水通道蛋白/生物信息学/非生物胁迫/表达分析Key words
potato/aquaporins/bioinformatics/abiotic stress/expression analysis引用本文复制引用
满全财,孟姿诺,李伟,蔡心汝,苏润东,付长青,高顺娟,崔江慧..马铃薯AQP基因家族鉴定及表达分析[J].生物技术通报,2024,40(9):51-63,13.基金项目
河北省承德市科技计划项目(202305B040),河北省脱毒马铃薯繁育技术创新中心(SG2012016),河北(承德)马铃薯种薯产业技术研究院创新能力提升(202206F013) （202305B040）
