生物技术通报2024，Vol.40Issue(9)：64-73,10.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0133
马铃薯SAT基因家族的鉴定和表达分析
Identification and Expression Analysis of SAT Gene Family in Potato(Solanum tuberosum L.)
摘要
Abstract
[Objective]Serine acetyltransferase(SAT)is a critical enzyme in assimilating sulfur into cysteine(Cys),participating in various biological processes in plants,especially in plant response to abiotic stress.However,analyses on the potato SAT gene family(StSAT)has not yet be reported.This study systematically and comprehensively identified the potato SAT gene family,providing a theoretical basis for further understanding the characteristics of the StSAT gene family and further analysis of their functions in potato resistance to abiotic stress.[Method]HMM was used to identify the StSAT gene family in potatoes and analyze their chromosomal distribution,gene structure,protein-conserved motifs,and collinearity across species.Additionally,RNA-seq data downloaded from PGSC were used to analyze the expression patterns of StSATs in different tissue parts of doubled-monoploid(DM)potatoes under abiotic stress and exogenous hormone treatments.qPCR(quantitative real-time PCR)was to analyze the relative expressions of StSATs in tetraploid potatoes under NaCl and PEG treatment(0,1,3,and 24 h)[Result]Four StSATs were identified in potatoes,and they were distributed across four chromosomes.Based on phylogenetic characteristics,these four StSATs were classified into three subfamilies.According to synteny analysis,there were 4 orthologous SAT genes to Arabidopsis,4 to tomato(Solanum lycopersicum),2 to cabbage(Brassica oleracea),1 to rice(Oryza sativa),and 1 to corn(Zea mays).Expression analysis showed that the expressions of all four StSATs significantly increased over time under NaCl and PEG treatments compared to 0 h,indicating their likely involvement in the potato's response to salt and osmotic stress.[Conclusion]StSAT gene family members play an essential role in potato responses to salt and osmotic stress.关键词
马铃薯/SAT基因家族/顺式作用元件/非生物胁迫/表达分析Key words
potato/SAT genes family/cis-acting element/abiotic stress/expression analysis引用本文复制引用
申鹏,高雅彬,丁红..马铃薯SAT基因家族的鉴定和表达分析[J].生物技术通报,2024,40(9):64-73,10.基金项目
甘肃省教育厅高校教师创新基金项目(2023B-379) （2023B-379）
