生物技术通报2024，Vol.40Issue(9)：74-81,8.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0441
木薯SDH蛋白的序列分析及其与MeH1.2关系的研究
Sequence Analysis of MeSDH Protein and Its Relationship with MeH1.2 in Cassava
摘要
Abstract
[Objective]Sorbitol dehydrogenase(SDH)plays an important role in regulating the conversion between fructose and sorbitol in Roseviaceae plants,and also participates in plant resistance to stress.The study on the function of MeSDH gene may provide a theoretical basis for cultivating cassava germplasm with high yield and resistance to stress.[Method]MeSDH gene was cloned using'SC124'cDNA as template,and the tissue specificity of MeSDH gene and its response pattern to drought,low temperature,PEG and ABA were analyzed by quantitative real-time PCR(RT-qPCR).MeH1.2 was used as bait to screen the mixed yeast cDNA library of cassava under drought and low temperature.Yeast two hybridization(Y2H)point-to-point and bimolecular fluorescence complementation(BiFC)experiments were further used to confirm the relationship between target proteins.[Result]The CDS length of MeSDH gene cloned from'SC124'cDNA is 1092 bp,encoding 364 amino acids,and the sequence shows no difference with it in the database.MeSDH protein contains catalytic Zn binding site,NADP binding site and structural Zn binding site,belonging to the MDR superfamily.MeSDH protein is localized in the nucleus by transforming tobacco leaves.The expression of MeSDH gene is decreasing in the order of functional leaves,young leaves,fibrous roots and stems.The expression of MeSDH is up-regulated in the cassava leaves treated with drought,low temperature and PEG,and significantly up-regulated in the cassava leaves and roots treated with ABA.Yeast library screening,Y2H point-to-point and BiFC experiments confirm the interaction between MeH1.2 and MeSDH.[Conclusion]MeSDH gene is up-regulated in response to various stresses,and probably MeSDH and MeH1.2 work together.关键词
木薯/SDH基因/MeH1.2蛋白/抗逆性/蛋白互作Key words
cassava/SDH gene/MeH1.2 protein/resistance to stress/protein interaction引用本文复制引用
赵平娟,林晨俞,王梦月,张秀春,李淑霞,阮孟斌..木薯SDH蛋白的序列分析及其与MeH1.2关系的研究[J].生物技术通报,2024,40(9):74-81,8.基金项目
海南省自然科学基金(321RC1095),国家现代农业产业技术体系-木薯(CARS-11-HNSHY),国家地区科学基金项目(3236045) （321RC1095）
