生物技术通报2024，Vol.40Issue(9)：82-91,10.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0460
彩色马铃薯花青素合成相关ERF基因筛选及表达分析
Screening and Expression Analysis of ERF Gene Related to Anthocyanin Synthesis in Colored Potato
摘要
Abstract
[Objective]Ethylene responsive factor(ERF)is an important transcription factor involved in the regulation of anthocyanin biosynthesis in plants.Screening of StERF genes that may be involved in the regulation of anthocyanin synthesis in potato tubers lays the foundation for the next step of research on the function of anthocyanin-related StERFs genes in colored potato.[Method]Bioinformatics methods were used to analyze their physicochemical properties,subcellular localization,conserved motifs,evolutionary relationships,protein secondary structure,promoter cis-acting elements,and protein interactions,while RT-qPCR was used to analyze the expression patterns of StERFs in potato flesh of different colors.[Result]Seven differentially expressed ERF transcription factors were obtained based on the transcriptome expression profiles of potato tubers of different colors.The seven StERFs are hydrophilic proteins,all of which are predicted to be localized in the nucleus.Motif 1(RWLG)and Motif 2(YRG)are the common conserved motifs in the seven StERFs,which belong to the structural domains of the characteristic sequences of AP2.The promoter sequences of the StERFs genes contain hormone-responsive action elements,as well as cis-action elements in response to defense and stress,low temperature and light,etc.The expressions of the seven StERFs were significantly higher in purple potato flesh than in yellow potato flesh,among which StERF72 and StERF110 were closer to known anthocyanin-associated ERFs(IbERF71 and PyERF3).The existence of protein interactions between the seven StERFs and 56 transcription factors.[Conclusion]Seven StERFs may be involved in the regulation of anthocyanin synthesis in potato tubers,among which StERF72 and StERF110 can be candidated genes to further validate their functions.关键词
彩色马铃薯/花青素/ERF转录因子/生物信息学分析/表达模式分析Key words
colored potato/anthocyanin/ERF transcription factor/bioinformatics analysis/expression pattern analysis引用本文复制引用
吴娟,武小娟,王沛捷,谢锐,聂虎帅,李楠,马艳红..彩色马铃薯花青素合成相关ERF基因筛选及表达分析[J].生物技术通报,2024,40(9):82-91,10.基金项目
内蒙古自治区马铃薯育种联合攻关项目(YZ2023006),内蒙古农业大学基本科研业务费专项(BR22-11-16),内蒙古自治区研究生科研创新项目(B20210182Z),内蒙古自治区高校基础科研专项基金项目(2022) （YZ2023006）
