马铃薯G6PDH基因家族鉴定及其在损伤块茎的表达分析OA北大核心CSTPCD

[Objective]Glucose-6-phosphate dehydrogenase(G6PDH)plays an important role in plant response to abiotic stress.Identification of the G6PDH gene family in potato and analysis of its expression pattern in damaged tubers would provide a reference for the subsequent in-depth study of the role of potato G6PDH genes in damage stress.[Method]We used bioinformatics methods to identify the G6PDH gene family members of the potato w,and to analyze the chromosomal distribution,protein physicochemical properties,secondary structure,evolutionary relationships,gene structure,conserved motifs,and promoter cis-acting elements of proteins encoded by members of the gene family,as well as the expression pattern in different organs and damaged tubers of potato.[Result]A total of 4 G6PDH family members in potato genome were identified and distributed on 4 chromosomes,named StG6PDH1-StG6PDH4.Based on the subcellular localization and phylogenetic analysis,StG6PDH1,StG6PDH3 and StG6PDH4 were located in the chloroplasts and belonged to the plastidic type,StG6PDH2 was located in the cytoplasm and belonged to the cytoplasmic type.The number of amino acids of potato G6PDH proteins ranged from 511 to 596 aa,molecular weights from 58.48 to 66.65 kD,isoelectric points from 5.83 to 8.57,and instabilities coefficient from 39.79 to 47.53.The secondary structure of protein showed the highest percentage of α-helices and irregular coils and the least of β-turns.In addition,the promoters of StG6PDHs contained a large number of phytohormone,light,and stress response elements.The four StG6PDHs were expressed in the roots,stems,leaves and tubers of potato,and the expression in the leaves was higher than in other organs.The members of StG6PDHs were involved in the responses of potato tubers to injury stress.The expressions of StG6PDH1,StG6PDH2 and StG6PDH3 were upregulated within 36 h after tubers injured,and StG6PDH4 was down-regulated in injured potato tubers.[Conclusion]A total of four potato StG6PDHs gene family members are identified,unevenly distributed on four chromosomes,of which one is cytoplasmic and three are plastidic.The promoter region of StG6PDHs includes light,hormone,and stress response elements.The expression of StG6PDHs in wounded tubers is differential,and the members synergistically regulate the responses of potato tubers to damage stress.