生物技术通报2024，Vol.40Issue(9)：198-211,14.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0269
辣椒UGT基因家族的鉴定及表达分析
Identification and Expression Analysis of UGT Gene Family in Pepper
摘要
Abstract
[Objective]The structure and function of UGT gene family members in pepper were identified and analyzed,laying the foundation for subsequent research.[Method]BioEdit,BLASTP and Pfam were used to align and search for UGT members in pepper.CDD and HMMER databases were used to verify conserved domains.ExPASy,Cell PLOC,MAGA X,MG2C,GSDS,and STRING were used to analyze and predict protein physicochemical properties,phylogeny,chromosome localization,gene structure and protein interaction.Transcriptome data and real-time PCR were used to analyze the expression pattern of UGT gene in various organs during the development of various organs.[Result]The prediction results showed that the 140 CaUGTs family members identified from the pepper gene family encoded amino acids in the range of 253-534 and the isoelectric point in the range of 4.7-8.45,most of the CaUGTs were located outside the cell,and a few were located in the plasma membrane and chloroplasts,and were distributed in 17 groups and mapped on 13 chromosomes,of which 25 members were located in chrom12,and all members contained the conserved domains motif 1 and motif 3.They responded to the action elements related to plant growth and development,stress.Real-time quantitative PCR analysis of the expression patterns of UGT gene in pepper under different tissues,stress and hormone response showed that UGT gene was of tissue-specific expression differences,and the expressionof UGT gene significantly increased or decreased under the conditions of ABA,GA3,high and low temperature stress,and CaUGTs members might play different roles in specific stages of development,and may be involved in regulating the defense response under stress in pepper.[Conclusion]The distribution and structure of the 140 pepper UGT members are diverse,while the members within the group are highly similar,indicating that the CaUGTs gene may respond to abiotic stress during the growth and development of pepper plants.关键词
辣椒/UGT基因家族/生物信息分析/成员鉴定/功能预测/表达分析Key words
pepper/UGT gene family/bioinformatics analysis/membership identification/functional prediction/expression analysis引用本文复制引用
吴慧琴,王延宏,刘涵,司政,刘雪晴,王静,阳宜,成妍..辣椒UGT基因家族的鉴定及表达分析[J].生物技术通报,2024,40(9):198-211,14.基金项目
山西省重点研发计划(202102140601014,202202140601006),山西省科技创新人才团队专项(20230451001018) （202102140601014,202202140601006）
