生物技术通报2024，Vol.40Issue(9)：212-224,13.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0276
基于转录组和WGCNA筛选大葱雄性不育相关基因
Screening Genes Related to Male Sterile in Welsh Onion(Allium fistulosum L.)Based on Transcriptomic Profiling and WGCNA
摘要
Abstract
[Objective]The aim of this study is to identify the co-expression modules of male sterile Welsh onion(Allium fistulosum L.)and to screen key hub genes,so as to provide deeper insights into the molecular mechanisms of male sterile in the Welsh onion.[Method]Twenty-four cDNA libraries were constructed and sequenced for different development stages of flower buds from the male-sterile(MS)plants and male-fertile(MF)plants.Then weighted gene co-expression network analysis(WGCNA)was performed to reveal the key modules and hub genes.[Result]A total of eighteen co-expression modules were identified based on 8,320 genes,among which three modules(blue,midnightblue,and black)were significantly correlated with pollen abortion of the MS plant.Functional enrichment analysis suggested that several pathways associated with the"phenylpropanoid biosynthesis""cutin,suberine,and wax biosynthesis""starch and sucrose metabolism""alpha-linolenic acid metabolism"and"fatty acid elongation"were found to be involved in pollen abortion of the MS plant.By considering gene significance and intramodular connectivity,several hub genes including atpB,TPS9,TPD1,and BHLH35,were identified,which might play key roles in pollen development of the Welsh onion.Then hub genes related to pollen development response were verified for quantitative real-time PCR(RT-qPCR)analysis,and the expression patterns of RNA-Seq and RT-qPCR were very consistent.[Conclusion]Three key modules of male sterile Welsh Onion are obtained,and hub genes like atpB,TPS9,and TPD1 are identified.It is found that the pollen abortion of the MS plant mainly is involved the processes of phenylpropanoid biosynthesis,cutin,suberine,and wax biosynthesis,starch and sucrose metabolism,alpha-Linolenic acid metabolism,and fatty acid elongation.关键词
大葱/雄性不育/加权基因共表达网络分析/转录组测序/核心基因Key words
Welsh onion/male sterile/weighted gene co-expression network analysis/RNA-Seq/hub gene引用本文复制引用
岳丽昕,王清华,刘泽洲,孔素萍,高莉敏..基于转录组和WGCNA筛选大葱雄性不育相关基因[J].生物技术通报,2024,40(9):212-224,13.基金项目
山东省重点研发计划(2023TZXD071),山东省蔬菜产业技术体系(SDAIT-05),山东省农业科学院农业科技创新工程(CXGC2024F08,CXG2023A07) （2023TZXD071）
