生物技术通报2024，Vol.40Issue(9)：270-281,12.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0050
复配菌群降解煤产甲烷的宏基因组与宏转录组分析
Metagenomic and Metatranscriptomic Analysis of Methanogenesis from Coal Degradation by Compounded Microflora
摘要
Abstract
[Objective]Aromatic compound metabolism is considered a limiting factor for the generation of biogenic coalbed methane.To increase biomethane production from coal,the compounded microflora(CM)was obtained by compounding raw inoculum(RI)with ability of producing methane by degrading coal and phenanthrene-degrading functional microflora enriched from produced water.[Method]A combined metagenome and metatranscriptome approach was used to analyze the differences in the community structure and metabolic pathways of CM and RI.[Result]The methane yield of CM increased significantly by 114.55%.Compounding significantly increased the proportion of aromatics-degrading bacteria,such as Pseudomonas,accounting for 63.49%,and also increased the metabolic activity of the dominant bacteria as well as the synthesis and expression of the key enzymes in the metabolic pathway of aromatic compounds.Gene abundance of the aromatics-degrading pathway in CM was 1.65 times higher than that of RI,and the gene expression abundance was 6.34 times higher than that of RI(P<0.05).The gene abundance and expression abundance of EC:1.13.11.2 were 2.24 and 62 times higher than those of RI,respectively.The increased expression abundance of these enzymes prompted the transformation of aromatic compounds to pyruvate.Compounding also enhanced the gene expression during the process of pyruvate metabolism to acetyl-CoA.The expression abundance of EC:1.2.4.1 reached 14.70-fold of the RI in CM.The gene expression abundance of the respective methanogenic pathways in CM was 2.66 to 7.10-fold of the RI.[Conclusion]The compounding enriched the aromatics-degrading bacteria and significantly increased gene abundance,especially gene expression abundance,in the whole metabolic pathway of degrading aromatics to methane generation,resulting in the increased methane production.关键词
煤层气/复配菌群/芳香族化合物/宏基因/宏转录Key words
coal bed methane/compounded microflora/aromatic compounds/metagenomic/metatranscriptomic引用本文复制引用
刘丁瑞,郭红光,弓凯仪..复配菌群降解煤产甲烷的宏基因组与宏转录组分析[J].生物技术通报,2024,40(9):270-281,12.基金项目
中国石油集团公司"十四五"前瞻性基础性技术攻关课题(2021DJ2302),国家自然科学基金项目(U181010351404163),山西省重点研发计划(国际合作 201903D421088) （2021DJ2302）
