RNA m1A和m5C甲基化修饰在拟轮枝镰孢伏马毒素生物合成中的作用OA北大核心CSTPCD

[Objective]Fusarium verticillioides is a serious plant pathogen fungus,which greatly reduces grain yield.It also produces class 2B carcinogen fumonisin,which threatens human and animal health.To explore the relationship between RNA methylation modification and fumonisin,the role of RNA methylated transferase in fumonisin synthesis was analyzed.[Method]HPLC was applied to determine the fumonisin synthesis ability of F.verticillium strains from different regions.Six kinds of RNA methylation modification detection methods,including m6A,mlA,m5C,Gm,m7G,and Um,were established by QuEChERS pretreatment combined with ultra-high performance liquid chromatograph-tandem mass spectrometry(UPLC-MS/MS).Then the RNA methylation modifications of different toxin-producing fungi were detected,and the RNA methylation modification genes related to fumonisin synthesis were identified by bioinformatics and RT-qPCR.[Result]It was found that there were significant differences in fumonisin synthesis ability of F.verticillium strains in different regions,the detection method for RNA methylation modification was successfully constructed,the methylation of RNA mlA and m5C was negatively correlated with fumonisin synthesis,and RT-qPCR showed that Fvalyref gene negatively regulated fumonisin biosynthesis.[Conclusion]The methylation modification of RNA m5C and its Reader gene Fvalyref negatively regulate fumonisin biosynthesis.