山西大学学报（自然科学版）2024，Vol.47Issue(5)：1097-1107,11.DOI:10.13451/j.sxu.ns.2024113
基于网络药理学探讨栀子苷抗结肠癌的作用机制
To Explore the Mechanism of Geniposide Against Colon Cancer Based on Network Pharmacology
摘要
Abstract
Geniposide is the main bioactive ingredient of traditional Chinese medicine Gardenia jasminoides.It has attracted substan-tial attention due to its pharmacological activities such as anti-inflammatory,antibacterial,hypoglycemic,antidepressant,antioxi-dant,immune modulation,and anti-tumor.However,the target and underlying mechanism of geniposide in colon cancer(CRC)cells remained elusive.Herein,we mainly screened the common targets of geniposide and CRC via network pharmacology,and construct-ed the target protein interaction network,and utilized the R package to perform enrichment analysis of biological functions(GO)and pathways(KEGG).The results indicated that the anti-CRC effect of geniposide is closely associated with cellular senescence.Drug affinity responsive target stability(DARTS)combined with mass spectrometry analysis was conducted to explore the potential tar-gets of geniposide in regulating CRC cell senescence.Moreover,molecular docking was utilized to predict the specific binding sites of geniposide and target proteins.We revealed that geniposide exerted anti-CRC effects by directly targeting FKBP prolyl isomerase 1A(FKBP1A).Cell function experiments further confirmed that geniposide significantly induced CRC cell senescence.Altogether,these results demonstrated that geniposide may trigger CRC cell senescence by targeting FKBP1A,which provides a theoretical ba-sis and reference for the research and development of geniposide for CRC treatment.关键词
栀子苷/结肠癌/细胞衰老/网络药理学/作用靶点/分子对接Key words
geniposide/colon cancer/cellular senescence/network pharmacology/targets/molecular docking分类
生物科学引用本文复制引用
李玉,尚琳琳,冯若琳,杨鹏,李宗伟..基于网络药理学探讨栀子苷抗结肠癌的作用机制[J].山西大学学报（自然科学版）,2024,47(5):1097-1107,11.基金项目
国家自然科学基金(82270217) （82270217）
