现代畜牧科技Issue(9)：5-8,4.DOI:10.19369/j.cnki.2095-9737.2024.09.002
猪德尔塔冠状病毒S蛋白纳米颗粒的构建及免疫原性评价
Construction and Immunogenicity Evaluation of Porcine deltacoronavirus Spike Protein Nanoparticles
摘要
Abstract
To evaluate the immunogenicity of Porcine deltacoronaviurs(PDCoV)spike(S)proteinnanoparticles,the recombinant proteins S1-CTD-Spy-His and SpyCatcher-mi3 were firstly expressed by using pcDNA3.1(+)-S1-CTD-Spa-His and pET28a-SpyCatcher-mi3,After purification,SDS-PAGE identification showed that there were target bands at 55 ku and 37 ku.Utilizing the SpyTag-SpyCatcher protein ligase system,S1-CTD-Spycatcher-mi3 nanoparticles were obtained,and the diameter of 35～40 nm nanoparticles were observed under electron microscopy.The results of serum antibody assay showed that S1-CTD-SpyCatcher-mi3 nanoparticles induced a strong humoral immune response in mice,and the specific IgG antibody levels of the S1-CTD-SpyCatcher-mi3 group were significantly higher than those of the S1-CTD group(P＜0.05),and the differences between the S1-CTD-SpyCatcher-mi3 group and the S1-CTD group compared with the PBS control group were both highly significant(P＜0.01).This study shows S1-CTD-SpyCatcher-mi3 nanoparticles have good immunogenicity and can induce immune protection in mice,which lays a foundation for the development of novel Porcine deltacoronavirus infection vaccine.关键词
冠状病毒/连接酶/纳米颗粒/小鼠/免疫原性Key words
coronavirus/ligase/nanoparticle/mouse/immunogenicity分类
农业科技引用本文复制引用
张晓蓉,吴建晓,高銘泽,高娜,陈建飞,冯力..猪德尔塔冠状病毒S蛋白纳米颗粒的构建及免疫原性评价[J].现代畜牧科技,2024,(9):5-8,4.基金项目
国家重点研发计划项目(No.2021YFD1801105) （No.2021YFD1801105）
中国农业科学院科技创新工程科学中心重点任务(CAAS-CSLPDCP-202301) （CAAS-CSLPDCP-202301）
