摘要
Abstract
In order to identify the serotypes,pathogenicity and drug resistance of sheep Pasteurella multocida in Miyun District,Beijing City,nasal swabs from sheep with respiratory diseases as well as lungs,livers and other lesion tissue samples from dead sheep were collected from free-range and intensive farms to isolate and identify Pasteurella multocida using bacterial isolation and culture,biochemical identification and PCR assay,and to identify the pathogenicity,serotypes and drug resistance of the isolates.The results showed that 98 strains of Pasteurella multocida were isolated,including 68 virulent strains that were divided into 3 serotypes including 54.4%serotype A,32.4%serotype B and 13.2%serotype D;and were 91.2%-100%resistant to 7 drugs such as amoxicillin,ampicillin and sulfadoxine mesoxymethane,but less than 20%only to ceftiofur and ceftriaxone.In conclusion,sheep respiratory diseases were mainly caused by Pasteurella multocida that was one of the major pathogens in the region,with serious drug resistance,and serotype A strain was dominant.Farms/households were thus recommended to improve their feeding management to reduce potential stimulation of stress factors to sheep,and to prevent and control diseases using sensitive drugs.关键词
多杀性巴氏杆菌/致病性/荚膜血清型/耐药性Key words
Pasteurella multocida/pathogenicity/capsular serotype/drug resistance分类
农业科技
