中国动物检疫2024，Vol.41Issue(9)：66-70,5.DOI:10.3969/j.issn.1005-944X.2024.09.014
施马伦贝格病诊断技术研究概况
Research Progress on Diagnostic Techniques for Schmallenberg Disease
摘要
Abstract
Schmallenberg disease(SBD)is an insect-borne infectious disease of ruminants caused by Schmallenberg virus(SBV),which should be prevented and controlled by confirmation through detecting pathogens and antibodies in time as there has been no any effective treatment methods.SBD is mainly diagnosed by etiological detection methods such as virus isolation and identification,RT-PCR and real-time fluorescent RT-RAA,as well as serological detection methods such as viral neutralization test(VNT),plaque reduction neutralization test(PRNT),enzyme-linked immunosorbent assay(ELISA)and immunochromatographic strips.Each of which has advantages and disadvantages.To be specific,virus isolation and identification and VNT are classical but time-consuming due to strict operation involving virus culture;RT-PCR and ELISA are simple and commonly used for SBV laboratory testing;and RT-RAA and immunochromatographic strips are suitable for rapid on-site screening with their short reaction time.Therefore,SBD should be diagnosed by one or more methods according to actual conditions,requirements and purposes.Here,the research progress of etiological and serological diagnosis technologies for SBD in recent years was summarized,aiming to provide a technical reference for monitoring,prevention and control of SBD.关键词
施马伦贝格病毒/诊断技术/病原学检测/血清学检测Key words
SBV/diagnostic technique/etiological detection/serological detection分类
农业科技引用本文复制引用
魏方,陈冬杰,王晶晶,余儒洋,王彩霞,冯春燕,于浩洋,吴绍强..施马伦贝格病诊断技术研究概况[J].中国动物检疫,2024,41(9):66-70,5.基金项目
"十四五"国家重点研发计划项目(2022YFD1800503,2022YFD1800505) （2022YFD1800503,2022YFD1800505）
