虾肝肠胞虫芯片式数字PCR定量检测方法的建立及应用OA
Establishment and Application of a cdPCR Assay for Enterocytozoon hepatopenaei
虾肝肠胞虫(Enterocytozoon hepatopenaei,EHP)感染是目前对虾行业面临的全球性挑战,在过去 10年中其发病率急剧上升.为建立一种可绝对定量检测EHP的方法,根据GenBank中EHP胞壁蛋白SWP基因保守区域,设计一套特异性引物和荧光探针,通过优化反应条件,建立了EHP芯片式数字PCR(chip digital PCR,cdPCR)定量检测方法.结果显示:该方法线性关系良好(R2=0.998 1);敏感性高,检测限可达…查看全部>>
Enterocytozoon hepatopenaei(EHP)infection has challenging global shrimp industry with a quick increasing prevalence over the past decade.In order to establish an absolute quantitative method for EHP,a set of specific primers and fluorescent probes were designed based on the conserved region of EHP SWP gene registered in GenBank,and a chip digital PCR(cdPCR)for EHP was established through optimizing reaction conditions.The results showed that the method had e…查看全部>>
陈玉红
福建省水产技术推广总站,福建福州 350002
水产学
虾肝肠胞虫芯片式数字PCR方法建立性能评估
EHPcdPCRmethod establishmentperformance evaluation
《中国动物检疫》 2024 (9)
105-110,6
2024年福建省水生动物疫病监测与控制项目福建省海洋服务与渔业高质量发展专项资金项目(FJHY-YYKJ-2024-3-6)
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