摘要
Abstract
Objective To investigate the effect of long non-coding RNA(LncRNA)Lnc-H2AFV-1 on the proliferation,migration,invasion and cisplatin resistance of head and neck squamous cell carcinoma(HNSCC)cells by regulating the expression of SEC11 homolog A(SEC11A).Methods CAL-27 cells were divided into NC group(transfected with si-NC),si-H2AFV-1 group(knocked down Lnc-H2AFV-1),cotransfection group(knocked down Lnc-H2AFV-1+overexpressed SEC11A).The relative expression level of SECl 1A mRNA in each cell line and cell group was detected by real-time fluorescence quantitative polymerase chain reaction.The relative expression level of SEC11A protein in each group was detected by Western blotting.The proliferation rate of each group was detected by 5-ethynyl-2'-deoxyuridine assay.The invasion ability of each group was evaluated by Transwell chamber assay.The migration ability of each group was detected by cell scratch assay.The resistance of each cell group to cisplatin was detected by cell counting kit-8 assay.Results The relative expression levels of SEC1 1A mRNA in the NC group and the si-H2AFV-1 group were 1.00±0.10 and 0.43±0.06,respectively;the relative expression levels of SEC11 A protein were 1.00±0.13 and 0.36±0.04,respectively.The differences between the two groups were statistically significant(all P＜0.05).The cell proliferation rates of NC group,si-H2AFV-1 group and cotransfection group were(43.73±3.68)％,(10.82±1.75)％and(51.66±4.91)％,respectively;the cell migration ratios were(43.51±4.63)％,(13.76±2.39)％and(54.29±5.91)％,respectively;the number of cells passing through the chamber was(189.65±18.27),(65.42±5.48)and(196.44±19.86),respectively.The half maximal inhibitory concentration(IC50)were 12.61,5.37 and 14.76 μg·mL-1,respectively.The above indexes in the si-H2AFV-1 group were statistically significant compared with the NC group and the cotransfection group(all P＜0.05).Conclusion Lnc-H2AFV-1 can promote the proliferation,migration,invasion and cisplatin resistance of HNSCC cells by regulating SEC11A expression.关键词
长链非编码RNA/头颈鳞癌/SEC11同源物A/舌鳞癌/增殖/迁移/顺铂耐药性Key words
long non-coding RNA/head and neck squamous cell carcinoma/SEC 11 homolog A/tongue squamous cell carcinoma/proliferation/migration/cisplatin resistance分类
医药卫生
