中国临床药理学杂志2024，Vol.40Issue(19)：2880-2884,5.DOI:10.13699/j.cnki.1001-6821.2024.19.024
川芎嗪调控创伤后应激障碍小鼠认知功能的作用
Effects of ligustrazine on cognitive function in mice with post-traumatic stress disorder
摘要
Abstract
Objective To investigate the effect and mechanism of tetramethylpyrazine(TMP)on cognitive function in mice with post-traumatic stress disorder(PTSD).Methods The mice were randomly divided into normal group,model group and experimental group.Except for the normal group,the PTSD mouse model was established by single prolonged stress(SPS).The experimental group was intraperitoneally injected with 10 mg·kg-1 TMP,and the normal group and the model group were intraperitoneally injected with an equal amount of 0.9％NaCl.The Morris water maze,open field and elevated plus maze tests were used to evaluate the cognitive behavior of the mice.The apoptosis of neurons was detected by Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL).The expression of ionized calcium binding adapter molecule-1(Iba-1)protein was detected by immunofluorescence(ICC).The content of oxidative stress inflammatory factors was detected by enzyme-linked immunosorbent assay(ELISA).Results The escape latency of the normal group,model group,and TMP group were(56.50±9.89),(87.16±10.48)and(68.63±10.19)s,respectively;the corner residence time of the open field were(190.37±40.64),(260.39±40.54)and(218.63±38.27)s,respectively;the apoptosis rates were(18.28±2.35)％,(39.36±3.65)％and(30.74±3.58)％,respectively;the fluorescence intensities of Iba-1 were(8.01±2.23)％,(50.87±7.31)％and(7.49±1.41)％;malondialdehyde contents were(5.46±0.95),(12.98±2.06)and(8.31±1.28)nmol·mg-1,respectively;tumor necrosis factor-α contents were(53.59±9.91),(115.46±11.53)and(74.38±10.77)pg·mL-1,respectively.The above indexes in the normal group and the experimental group were statistically significant compared with the model group(P＜0.05,P＜0.01).Conclusion TMP can improve the cognitive function of PTSD mice,and the mechanism may be related to the regulation of inflammation and oxidative stress.关键词
川芎嗪/创伤后应激障碍/认知功能/炎症反应/氧化应激Key words
tetramethylpyrazine/post-traumatic stress disorder/cognitive function/inflammatory response/oxidative stress分类
医药卫生引用本文复制引用
郭玲,陈永权,刘灿,姚卫东,姚悦,承萍萍,柳兆芳..川芎嗪调控创伤后应激障碍小鼠认知功能的作用[J].中国临床药理学杂志,2024,40(19):2880-2884,5.基金项目
安徽省自然科学基金资助项目(2208085MC55) （2208085MC55）
皖南医学院校中青年科研基金资助项目(WK2021F12) （WK2021F12）
