中国临床药理学杂志2024，Vol.40Issue(19)：2891-2895,5.DOI:10.13699/j.cnki.1001-6821.2024.19.026
基于UPLC-QTOF/MS的非靶向代谢组学构建2型糖尿病患者血浆代谢轮廓的研究
Construction of plasma metabolic profile of type 2 diabetic mellitus patients based on UPLC-QTOF/MS non-targeted metabonomics
摘要
Abstract
Objective To explore the changes of plasma metabolites in Uygur and Kazak patients with type 2 diabetes mellitus(T2DM)and people with normal glucose tolerance(NGT),construct the profile of different metabolites and analyze different metabolic pathways.Methods Plasma samples of T2DM and NGT from Uygur and Kazak were collected for detection.Ultra-high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry was used for detection.MetaboAnalyst 5.0 was used to analyze the data,and the screening conditions were P＜0.05 and fold change(FC)＜0.90 or FC＞1.10.We used The Kyoto Encyclopedia of Genes and Genomes database to enrich and explore the metabolic pathways of different metabolites.Results Compared with the Uighur NGT group,there are 109 differential metabolites in the T2DM group,and 43 unique differential metabolites are mainly concentrated in five pathways,such as phenylalanine biosynthesis,tyrosine biosynthesis,and tryptophan metabolism.Compared with the NGT group,Kazak T2DM has 86 differential metabolites,and 28 unique differential metabolites are mainly enriched in the urea cycle,arginine,and proline metabolism(impact＞0.10,P＜0.05).Conclusion In this study,the plasma metabolic profile of T2DM patients was constructed,and the unique metabolites of T2DM in Uygur and Kazak were screened out,which provided potential biomarkers for early prevention of T2DM.关键词
超高效液相色谱飞行时间质谱/2型糖尿病/维吾尔族/哈萨克族/非靶向代谢组学Key words
ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry/type 2 diabetes millitus/Uygur/Kazak/non-targeted metabonomics分类
医药卫生引用本文复制引用
朱曼丽,范雁东,王烨,姬凤彩,杨雪,李雪卉,李琳琳..基于UPLC-QTOF/MS的非靶向代谢组学构建2型糖尿病患者血浆代谢轮廓的研究[J].中国临床药理学杂志,2024,40(19):2891-2895,5.基金项目
国家自然科学基金地区基金资助项目(82360728) （82360728）
国家自然科学基金新疆联合基金本地优秀青年人才培养专项基金资助项目(U1403322) （U1403322）
