中国农业科技导报2024，Vol.26Issue(10)：71-82,12.DOI:10.13304/j.nykjdb.2024.0112
不同授粉强度对蓝莓柱头响应机制的转录组分析
Transcriptome Analysis of Stigma Response Mechanism of Blueberry with Different Pollination Intensity
摘要
Abstract
Pollination is essential for blueberry production.In order to explore the stigma response mechanism and its characteristics under different pollination intensities,the stigma acceptability of'Bluesouth(M7)'was compared at different florescence ages,and the pollen viability was determined.Transcripome sequencing and analysis were performed on the stigma of blueberry under 3 pollination intensities.The results showed that,with the increasing of flowering age,stigma receptivity of'Bluesouth(M7)'showed a trend of increasing first and then decreasing gradually,with the strongest receptivity on the second day of flowering.Pollen from 3 different pollination intensities showed different vigor.Blueberry stigma transcriptome data analysis revealed that with the increase in pollination intensity,the expression levels of gene CPK17 and gene MIOX1 were significantly upregulated.The GO(gene ontology)enrichment analysis of the differentially expressed genes showed that the genes involved in carbohydrate metabolic process,inositol oxygenase activity and cell wall organization were significantly enriched.KEGG(kyoto encyclopedia of genes and genomes)enrichment analysis showed that the pathways related to plant-pathogen interaction and inositol phosphate metabolism were significantly enriched.It was inferred that the increase of pollination intensity might affect the germination and growth of pollen tube,and then affect the fertilization process.These findings suggested that increased pollination intensity might impact the process of stigma and pollen recognition,and facilitate pollen tube growth through involvement in signal transduction and hormone synthesis between stigma and pollen.Above results provided a theoretical reference for understanding the mechanism of stigma response to different pollination intensities of blueberry,and provided scientific basis for precise pollination of blueberry.关键词
蓝莓/柱头/授粉强度/转录组测序/差异表达基因Key words
blueberry/stigma/pollination intensity/transcriptome sequencing/differentially expressed genes分类
农业科技引用本文复制引用
冀薇,姚军,樊莹,黄家兴,杨慧鹏,徐进,李小英,郭岳琴,吴跃国,李继莲..不同授粉强度对蓝莓柱头响应机制的转录组分析[J].中国农业科技导报,2024,26(10):71-82,12.基金项目
中国农业科学院农业科技创新项目(CAAS-ASTIP-2023-IAR) （CAAS-ASTIP-2023-IAR）
国家蜂产业技术体系建设项目(CARS-44-KSJ5) （CARS-44-KSJ5）
云南省李继莲专家工作站(No.202105AF150072) （No.202105AF150072）
中国农业科学院基本科研业务费专项(MFSJKF2022-06). （MFSJKF2022-06）
