中国农业科技导报2024，Vol.26Issue(10)：83-97,15.DOI:10.13304/j.nykjdb.2024.0365
小粒咖啡叶绿素合成基因CaPOR的全基因组鉴定及表达分析
Genome-wide Identification and Expression Analysis of Key Chlorophyll Synthesis Related Gene CaPOR in Coffea arabica
摘要
Abstract
Chlorophyll plays a pivotal role in the process of photosynthesis and the accumulation of organic substances in plants.Pro tochlorophyllide oxidoreductase(POR)is an enzyme that catalyzes the conversion of prochlorophyllide,the precursor of chlorophyll,into chlorophyll a in the chloroplast.It is a critical enzyme for the light-induced greening of angiosperms and is indispensable for photosynthesis.To explore the genes involved in chlorophyll synthesis in Coffea arabica leaves,the transcriptome sequencing analysis was used to identify the chlorophyll synthesis-related genes in C.arabica.Furthermore,the real-time fluorescence quantitative analysis was used to examine the expression of key genes.The results showed that CaPOR gene family members shared similar physicochemical properties and conserved motifs.The promoter regions were primarily composed of light response elements and growth and development elements.Through a combination of chromosome localization,collinearity analysis,and codon usage bias analysis,CaPOR gene had formed a gene cluster in C.arabica,and there was an acceleration of gene duplication,which might be driven by the base mutation and natural selection.The expression levels of 7 selected CaPOR genes were markedly higher in mature leaves than those in young leaves,which was positively correlated with the change in chlorophyll synthesis during the growth and development of C.arabica leaves.Above results provided reference for the chlorophyll synthesis in C.arabica leaves and the functional exploration of POR genes.关键词
小粒咖啡/叶绿素/生物信息学分析/表达分析Key words
Coffea arabica/chlorophyll/bioinformatic analysis/expression analysis分类
农业科技引用本文复制引用
张振伟,邱俊超,陈治华,葛宇,董相书,杨婧,李学俊,杞美军,蒋快乐,杨永林,王步天,施学东..小粒咖啡叶绿素合成基因CaPOR的全基因组鉴定及表达分析[J].中国农业科技导报,2024,26(10):83-97,15.基金项目
云南省创新引导与科技型企业培育计划乡村振兴科技专项(202304BI090006) （202304BI090006）
云南省"兴滇英才支持计划"青年人才专项(XDYC-QNRC-2022-0711) （XDYC-QNRC-2022-0711）
云南省基础研究计划面上项目(202301AT070493) （202301AT070493）
云南省重点研发计划项目(202303AP140010) （202303AP140010）
云南省专家基层科研工作站项目(2021RYZJGZZ002) （2021RYZJGZZ002）
云南农业大学本科实践教学、劳动教育和耕读教育教学改革研究与实践项目(2024SJJX306). （2024SJJX306）
