中国农业科技导报2024，Vol.26Issue(10)：98-109,12.DOI:10.13304/j.nykjdb.2024.0022
基于转录组和代谢组解析川芎对镉胁迫的响应机制
Analysis of Response Mechanism of Ligusticum chuanxiong to Cadmium Stress Based on Transcriptome and Metabolome
摘要
Abstract
In order to understand the changes of gene expression and metabolites of Ligusticum chuanxiong under different levels of cadmium(Cd)stress,and explore the response mechanism of Ligusticum Chuanxiong to Cd stress,a pot experiment was conducted,and 4 Cd stress treatment levels of 1,3,6,9 mg·kg-1 were set with clean soil without adding Cd solution as control group,and RNA sequencing(RNA-seq)and ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)were used to identify key genes and metabolic pathways for resistance to Cd stress in Ligusticum chuanxiong.The results showed that a total of 8 569 differentially expressed genes were screened,including 6 859 up-regulated genes and 1 710 down-regulated genes.Compared with the control group,there was only 1 common differential gene in the 4 Cd-treated groups,CML19,which might be the key gene for resistance to Cd stress in Ligusticum chuanxiong.The metabolome labeled and quantized 1 238 different metabolites,and 76 metabolic pathways were annotated in the KEGG database,including amino acid metabolism,biosynthesis of aminoacyl-tRNA and biosynthesis of fatty acids.The joint analysis showed that Ligusticum chuanxiong played an important role in the resistance to Cd stress by regulating different amino acids to maintain its own metabolic balance when subjected to different levels of Cd stress.Above results provided scientific support and theoretical basis for the resistance of Ligusticum chuanxiong to Cd stress,and provided a basis for the subsequent cultivation and breeding of Ligusticum chuanxiong resources and related research on alleviating Cd stress.关键词
川芎/镉胁迫/转录组/代谢组Key words
Ligusticum chuanxiong/cadmium stress/transcriptome/metabolome分类
农业科技引用本文复制引用
徐皖菁,徐正君,张超,彭芳,赵豆豆,罗姣姣,陶珊,廖海浪,毛常清,吴宇,朱秀..基于转录组和代谢组解析川芎对镉胁迫的响应机制[J].中国农业科技导报,2024,26(10):98-109,12.基金项目
四川省育种攻关项目(2021YFYZ0012) （2021YFYZ0012）
四川省财政自主创新专项(2022ZZCX076). （2022ZZCX076）
