中国农业科学2024，Vol.57Issue(18)：3601-3611,11.DOI:10.3864/j.issn.0578-1752.2024.18.007
脂肪酸类天然产物2E,4E-癸二烯酸对烟草根际微生物群落的影响
Effect of Fatty Acid Natural Product 2E,4E-Decadienoic Acid on Tobacco Rhizosphere Microbial Communities
摘要
Abstract
[Objective]The fatty acid compound 2E,4E-decadienoic acid(DDA)was obtained from coculture of Bacillus subtilis Tpb55 and Trichoderma asperellum HG1,which has a specific inhibitory effect on Phytophthora.The objective of this study is to evaluate the effects of DDA on tobacco black shank(caused by P.nicotianae)and on the microbial community of tobacco rhizosphere soil,and to provide a basis for the application of such natural products in the prevention and control of plant Phytophthora disease.[Method]To evaluate the control efficacy of DDA against tobacco black shank,a pot experiment was performed,and the DNA copies of P.nicotianae in tobacco rhizosphere soil were detected by real-time fluorescence quantitative PCR technique.The bacterial,fungal and oomycetal communities in tobacco rhizosphere soil were analyzed through Illumina Hiseq high-throughput sequencing,and the correlation between soil nutrient factors and microbial community structure was assayed based on redundancy analysis and Mantel test.[Result]Compared with the control treatment with 0.5%DMSO,the treatment with 1.25 g·L-1 DDA for root irrigation showed 77.06%and 84.35%decrease for black shank disease index and P.nicotianae DNA copy number,respectively.DDA treatment also significantly increased the soil pH from 6.72 to 6.82,improved the soil conductivity by 102.11%,and decreased the soil organic matter by 13.05%.DDA treatment had no significant effect on the diversity and richness of fungi and bacteria in tobacco rhizosphere soil.However,the OTU level,Shannon index,Chao1 index,and ACE index of oomycetes significantly increased,while the Simpson index significantly decreased.The PCoA results showed that DDA treatment significantly affected the structure of tobacco rhizosphere oomycete community(r=0.667,P=0.028),but had no significant effect on fungi(r=0.259,P=0.305)and bacteria(r=0.593,P=0.098).The relative abundance of gram-positive bacterial genera such as Phycicoccus and Terrabacter significantly increased in DDA treatment,while the abundance of gram-negative bacterial genera including Devosia,Bordetella and Pseudoxanthomonas significantly decreased.Some beneficial fungi such as Aspergillus,Albifimbria,Arcopilus were enriched by DDA.For oomycetes,the relative abundance of Phytophthora was significantly decreased,but that of Globisporangium increased.The Mantel test results indicated that the changes in soil physicochemical properties caused by the application of DDA had no significant impact on the rhizosphere microbial community.[Conclusion]2E,4E-decadienoic acid(DDA),as a novel natural fatty acid compound,can effectively prevent and control tobacco black shank,reduce the pathogen DNA copy number in tobacco rhizosphere soil,and specifically regulate the structure of oomycete community.It has the application potential in the prevention and control of crop oomycete diseases.关键词
棘孢木霉/枯草芽孢杆菌/烟草黑胫病/共培养/2E,4E-癸二烯酸/根际微生物群落Key words
Trichoderma asperellum/Bacillus subtilis/tobacco black shank/coculture/2E,4E-decadienoic acid(DDA)/rhizosphere microbial community引用本文复制引用
彭治鑫,张希芬,韩小斌,司国栋,徐康文,张成省..脂肪酸类天然产物2E,4E-癸二烯酸对烟草根际微生物群落的影响[J].中国农业科学,2024,57(18):3601-3611,11.基金项目
山东省农业重大应用技术创新项目(SD2019ZZ002)、中国烟草总公司重大科技项目(110202101057(LS-17))、青岛市科技惠民示范专项(24-1-8-xdny-8-nsh) （SD2019ZZ002）
