不同配伍比例的半枝莲-白花蛇舌草药对对肝癌细胞增殖活性的影响OACSTPCD

Objective:To study the inhibitory effect of Scutellariae Barbatae Herba(SBH)and Hedyotis Diffusae Herba(HDH)couplet intervention with different compatibility ratio on the proliferation activity of liver cancer cell lines(MHCC97H cells and Huh7 cells).Methods:CCK-8 assay was used to observe the intervention of MHCC97H and Huh7 cells with different compatibility ratio of SBH and HDH(1∶1,2∶1,1∶2)for 24 h,and their IC50 values were calculated respectively.The cell plate clone formation experiment was used to detect the cell clone formation ability.The effects of different compatibility ratios of SBH and HDH on the proliferation activity of MHCC97H and Huh7 cells were analyzed.Results:The survival activity of MHCC97H and Huh7 cells significantly decreased(P<0.01)with different compatibility ratios of SBH and HDH in a concentration-dependent manner.The IC50 values of group 1∶1 were 6.69 mg/mL and 7.95 mg/mL,respectively;the IC50 values of group 2∶1 were 2.58 mg/mL and 3.43 mg/mL,respectively;and the IC50 values of group 1∶2 were 15.04 mg/mL and 16.60 mg/mL,respectively.The clone formation rate of MHCC97H and Huh7 cells was significantly reduced after intervention with different compatibility ratios of SBH and HDH(P<0.01).Different compatibility ratios of SBH and HDH have a significant inhibitory effect on the proliferation activity of MHCC97H and Huh7 cells.Conclusion:Different compatibility ratios of SBH and HDH can all inhibit the proliferation activity of liver cancer MHCC97H and Huh7 cells,and the best inhibition effect is achieved with a 2∶1 compatibility ratio,which can serve as an experimental basis for SBH and HDH couplet anti-tumor research.