中医药学报2024，Vol.52Issue(10)：16-24,9.DOI:10.19664/j.cnki.1002-2392.240197
黄芪甲苷通过调控TGF-β/Smad信号通路抑制胃癌的侵袭、迁移及上皮间质转化的研究
Study on Astragaloside Inhibiting the Invasion,Migration and Epithelial-Mesenchymal Transformation of Gastric Cancer by Regulating TGF-β/Smad Signaling Pathway
摘要
Abstract
Objective:To investigate the effects of astragaloside on the invasion,migration and the expression of epithelial mesenchymal transition(EMT)related molecules of gastric cancer through TGF-β/Smad signaling pathway.Methods:The effects of astragaloside on invasion and migration of gastric cancer cells were detected by scratch and transwell assay.The effects of astragaloside on mRNA and protein expression levels of key genes and EMT-related markers in TGF-β/Smad signaling pathway were detected through qRT-PCR and Western-blot,respectively.Results:The scratch test results showed that the healing rate of the two gastric cancer cells after the intervention of low concentration of astragaloside was significantly lower than that of the control group,the difference was statistically significant(P<0.05,P<0.01),and the healing degree gradually weakened with the increase of astragaloside concentration.The results of transwell migration and invasion experiment showed that the number of cells passing through the compartment after the intervention of astragaloside was significantly lower than that of the control group,and the difference was statistically significant(P<0.05,P<0.01).It was showed in qRT-PCR and Western-blot tests that the protein expression levels of TGF-β1,TGF-β2,TGF-βR2,P-SMad2,P-SMad3 and Smad4 in astragaloside group were significantly lower than those in control group(P<0.05,P<0.01).There were no significant differences in protein expression levels of Smad2 and Smad3 and mRNA levels of TGF-β1,TGF-β2,TGF-βR2,Smad2,Smad3 and Smad4 between astragaloside group and control group(P>0.05).The levels of a-SMA,Collagen 1,N-cadherin,Cytokinetin18,Claudin7,ZO-1,Vimentin,Snail1,Snail2,FN1,and mRNA,and expression levels of Twist2 mRNA and protein in astragaloside treatment group were lower than those in the control group(P<0.05,P<0.01),while the mRNA and protein levels of E-cadherin were higher than those in control group,and the difference was statistically significant(P<0.05,P<0.01).Conclusion:Astragaloside regulates EMT of gastric cancer cells through TGF-β/Smad signaling pathway,thereby inhibiting the invasion and migration of gastric cancer cells.关键词
黄芪甲苷/TGF-β/Smad/胃癌/EMT/侵袭/迁移Key words
Astragaloside/TGF-β/Smad/Gastric cancer/EMT/Invasion/Migration分类
医药卫生引用本文复制引用
陈凤琴,宁月,邵利华,王蒙,李海龙..黄芪甲苷通过调控TGF-β/Smad信号通路抑制胃癌的侵袭、迁移及上皮间质转化的研究[J].中医药学报,2024,52(10):16-24,9.基金项目
甘肃省自然科学基金项目(20JR5RA189) （20JR5RA189）
甘肃省科技厅联合科研基金一般项目(23JRRA1527) （23JRRA1527）
甘肃中医药大学附属医院院内创新基金项目(gzfy-2022-12) （gzfy-2022-12）
