北京中医药大学学报2024，Vol.47Issue(9)：1203-1210,8.DOI:10.3969/j.issn.1006-2157.2024.09.003
不同刺法对单纯性子宫内膜增生模型小鼠巨噬细胞极化影响的比较研究
Comparative study on the effects of different acupuncture technique on macrophage polarization in a mouse model of simple endometrial hyperplasia
摘要
Abstract
Objective We aimed to observe the differences in the effects of different acupuncture technique on the endometrium of mice with simple endometrial hyperplasia model and to explore the potential mechanisms. Methods According to the random number tables,32 female C57BL/6J mice were divided into a blank control group,a model group,a quick needle group and a retaining needle group,with 8 mice in each group. A mouse model of simple endometrial hyperplasia was established using bilateral ovariectomy combined with estrogen loading. In the quick needle group,mice were punctured at the bilateral for "Yinbai"(SP1) points and withdrawn immediately,with the treatmeat performed once every other day for a total of 12 times. In the retaining needle group,mice were punctured at the bilateral "Yinbai"(SP1) points and the needles were retained for 15 min each time,with the treatment also performed once every other day for a total of 12 times. After the intervention,samples were collected. HE staining was used to observe morphological changes in the mouse uterine tissue;ELISA was used to detect serum estradiol level;flow cytometry was used to detect the ratio of M1 and M2 macrophages(M1/M2) and immunohistochemical method was used to measure the expression of CD86 and CD206 in uterine tissue;and Western blotting was used to detect the expression of interleukin-13 (IL-13) and interferon-γ(IFN-γ) in uterine tissue. Results The endometrium of mice in the model group showed simple hyperplasia. Compared with the blank control group,the endometrium of the model group was thickened (P<0.01);the level of estradiol in the serum was increased (P<0.01);M1/M2 in uterine tissues was decreased (P<0.01),the expression of CD86 was decreased (P<0.01),and the positive expression of CD206 was increased (P<0.01);and the level of IFN-γ protein expression in uterine tissues was decreased (P<0.01),and the expression of IL-13 protein was increased (P<0.01). Compared with the model group,the endometrial thicknesses of the quick needle group and the retaining needle group were reduced (P<0.05),the levels of estradiol in serum were reduced (P<0.05),M1/M2 in uterine tissues increased (P<0.01),and the reduction of CD206 positive expression,and IL-13 protein expression reduced (P<0.01);the level of CD86 positive expression,IFN-γ protein expression increased (P<0.01). Compared with the quick needle group,IL-13 protein expression increased in the retaining needle group (P<0.01).Conclusion Both quick needle and retaining needle may be through the regulation of the expression of IFN-γ and IL-13,thus prompting the polarization of macrophages from M2 to M1 type,inhibiting the pro-cell proliferative ability and tissue repair ability of M2 type macrophages,thus reducing the degree of endometrial hyperplasia,and the quick needle group was superior to the retaining needle group in regulating the expression of IL-13.关键词
单纯性子宫内膜增生/隐白/巨噬细胞/巨噬细胞极化/快针/留针/小鼠Key words
simple endometrial hyperplasia/Yinbai(SP1)/macrophage/macrophage polarization/quick needle/retaining needle/mice分类
医药卫生引用本文复制引用
刘俊亚,李旭欣,张细芬,高渊博,佘延芬,李云峰,吕晶,郑文超,左广,王雪松,范玺胜,张俊茶,刘君..不同刺法对单纯性子宫内膜增生模型小鼠巨噬细胞极化影响的比较研究[J].北京中医药大学学报,2024,47(9):1203-1210,8.基金项目
国家自然科学基金项目(No.81973755,No.81971434) （No.81973755,No.81971434）
河北中医药大学创新资助项目(No.XCXZZSS2023015)National Natural Science Foundation of China(Nos. 81973755 and 81971434) （No.XCXZZSS2023015）
